TY - JOUR

T1 - Effects of different fractions of whey protein on postprandial lipid and hormone responses in type 2 diabetes

AU - Mortensen, L.S.

AU - Holmer-Jensen, Jens

AU - Hartvigsen, Merete

AU - Jensen, V.K.

AU - Astrup, Arne

AU - Vrese, M. de

AU - Holst, Jens Juul

AU - Thomsen, Claus Heikki Paavo

AU - Hermansen, Kjeld

N1 - IHE 2012 007

PY - 2012

Y1 - 2012

N2 - Background/Objectives:Exacerbated postprandial lipid responses are associated with an increased cardiovascular risk. Dietary proteins influence postprandial lipemia differently, and whey protein has a preferential lipid-lowering effect. We compared the effects of different whey protein fractions on postprandial lipid and hormone responses added to a high-fat meal in type 2 diabetic subjects.Subjects/Methods:A total of 12 type 2 diabetic subjects ingested four isocaloric test meals in randomized order. The test meals contained 100¿g of butter and 45¿g of carbohydrate in combination with 45¿g of whey isolate (iso-meal), whey hydrolysate (hydro-meal), a-lactalbumin enhanced whey (lac-meal) or caseinoglycomacropeptide enhanced whey (CGMP-meal). Plasma concentrations of triglyceride, retinyl palmitate, free fatty acid, insulin, glucose, glucagon, glucagon-like peptide 1 and glucose-dependent insulinotropic peptide were measured before and at regular intervals until 8-h postprandially.Results:We found no statistical significant differences between meals on our primary variable triglyceride. The retinyl palmitate response was higher after the hydro-meal than after the iso- and lac-meal in the chylomicron-rich fraction (P=0.008) while no significant differences were found in the chylomicron-poor fraction. The hydro- and iso-meal produced a higher insulin response compared with the lac- and CGMP-meal (P<0.001). Otherwise no significant differences in the hormone responses were found in the incremental area under the curve over the 480-min period.Conclusions:A supplement of four different whey protein fractions to a fat-rich meal had similar effects on postprandial triglyceride responses in type 2 diabetic subjects. Whey isolate and whey hydrolysate caused a higher insulin response.European Journal of Clinical Nutrition advance online publication, 16 May 2012; doi:10.1038/ejcn.2012.48.

AB - Background/Objectives:Exacerbated postprandial lipid responses are associated with an increased cardiovascular risk. Dietary proteins influence postprandial lipemia differently, and whey protein has a preferential lipid-lowering effect. We compared the effects of different whey protein fractions on postprandial lipid and hormone responses added to a high-fat meal in type 2 diabetic subjects.Subjects/Methods:A total of 12 type 2 diabetic subjects ingested four isocaloric test meals in randomized order. The test meals contained 100¿g of butter and 45¿g of carbohydrate in combination with 45¿g of whey isolate (iso-meal), whey hydrolysate (hydro-meal), a-lactalbumin enhanced whey (lac-meal) or caseinoglycomacropeptide enhanced whey (CGMP-meal). Plasma concentrations of triglyceride, retinyl palmitate, free fatty acid, insulin, glucose, glucagon, glucagon-like peptide 1 and glucose-dependent insulinotropic peptide were measured before and at regular intervals until 8-h postprandially.Results:We found no statistical significant differences between meals on our primary variable triglyceride. The retinyl palmitate response was higher after the hydro-meal than after the iso- and lac-meal in the chylomicron-rich fraction (P=0.008) while no significant differences were found in the chylomicron-poor fraction. The hydro- and iso-meal produced a higher insulin response compared with the lac- and CGMP-meal (P<0.001). Otherwise no significant differences in the hormone responses were found in the incremental area under the curve over the 480-min period.Conclusions:A supplement of four different whey protein fractions to a fat-rich meal had similar effects on postprandial triglyceride responses in type 2 diabetic subjects. Whey isolate and whey hydrolysate caused a higher insulin response.European Journal of Clinical Nutrition advance online publication, 16 May 2012; doi:10.1038/ejcn.2012.48.

KW - Aged

KW - Area Under Curve

KW - Cardiovascular Diseases

KW - Caseins

KW - Chylomicrons

KW - Diabetes Mellitus, Type 2

KW - Dietary Fats

KW - Dietary Proteins

KW - Dietary Supplements

KW - Female

KW - Glycopeptides

KW - Humans

KW - Hyperlipidemias

KW - Hypolipidemic Agents

KW - Insulin

KW - Lactalbumin

KW - Male

KW - Middle Aged

KW - Milk Proteins

KW - Postprandial Period

KW - Protein Hydrolysates

KW - Triglycerides

KW - Vitamin A

U2 - 10.1038/ejcn.2012.48

DO - 10.1038/ejcn.2012.48

M3 - Journal article

C2 - 22588635

VL - 66

SP - 799

EP - 805

JO - European Journal of Clinical Nutrition

JF - European Journal of Clinical Nutrition

SN - 0954-3007

IS - 7

ER -