Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue

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Standard

Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue. / Petersen, Ole William; Høyer, Poul Erik; van Deurs, Bo.

I: Virchows Archiv B Cell Pathology Including Molecular Pathology, Bind 50, Nr. 1, 12.1986, s. 13-25.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Petersen, OW, Høyer, PE & van Deurs, B 1986, 'Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue', Virchows Archiv B Cell Pathology Including Molecular Pathology, bind 50, nr. 1, s. 13-25. https://doi.org/10.1007/BF02889887

APA

Petersen, O. W., Høyer, P. E., & van Deurs, B. (1986). Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue. Virchows Archiv B Cell Pathology Including Molecular Pathology, 50(1), 13-25. https://doi.org/10.1007/BF02889887

Vancouver

Petersen OW, Høyer PE, van Deurs B. Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue. Virchows Archiv B Cell Pathology Including Molecular Pathology. 1986 dec.;50(1):13-25. https://doi.org/10.1007/BF02889887

Author

Petersen, Ole William ; Høyer, Poul Erik ; van Deurs, Bo. / Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue. I: Virchows Archiv B Cell Pathology Including Molecular Pathology. 1986 ; Bind 50, Nr. 1. s. 13-25.

Bibtex

@article{9ce184585ab349b9a58d4083d8f70fde,
title = "Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue",
abstract = "We here report a method for the characterization of carcinoma cells in cryosections of human breast tissue. Cryosections from 37 biopsies including carcinomas, fibrocystic disease specimens and reduction mammoplasties were incubated in an atmosphere of either pure nitrogen or 99.5% oxygen. The incubation medium contained neotetrazolium, phenazine methosulphate and the colloid stabilizer Polypep 5115. A marked overlap in formation of reaction product was found between carcinomas, benign tissue and normal breast tissue when sections were incubated in pure nitrogen. However, following 5 min of oxygen incubation no reaction was seen in normal breast tissue, whereas carcinoma cells consistently showed reaction. Moreover, a statistically significant difference was seen between the level of reaction in carcinoma cells and that of ductal epithelial cells in fibrocystic disease not containing apocrine metaplasia. Apocrine metaplasia, on the other hand, showed the highest activity recorded at all.",
keywords = "Glucose 6-phosphate dehydrogenase cytochemistry, Human breast tissue, Oxygen effect on tetrazolium reaction",
author = "Petersen, {Ole William} and H{\o}yer, {Poul Erik} and {van Deurs}, Bo",
year = "1986",
month = dec,
doi = "10.1007/BF02889887",
language = "English",
volume = "50",
pages = "13--25",
journal = "Virchows Archiv B Cell Pathology Including Molecular Pathology",
issn = "0042-6431",
publisher = "Springer Verlag",
number = "1",

}

RIS

TY - JOUR

T1 - Effect of oxygen on the tetrazolium reaction for glucose 6-phosphate dehydrogenase in cryosections of human breast carcinoma, fibrocystic disease and normal breast tissue

AU - Petersen, Ole William

AU - Høyer, Poul Erik

AU - van Deurs, Bo

PY - 1986/12

Y1 - 1986/12

N2 - We here report a method for the characterization of carcinoma cells in cryosections of human breast tissue. Cryosections from 37 biopsies including carcinomas, fibrocystic disease specimens and reduction mammoplasties were incubated in an atmosphere of either pure nitrogen or 99.5% oxygen. The incubation medium contained neotetrazolium, phenazine methosulphate and the colloid stabilizer Polypep 5115. A marked overlap in formation of reaction product was found between carcinomas, benign tissue and normal breast tissue when sections were incubated in pure nitrogen. However, following 5 min of oxygen incubation no reaction was seen in normal breast tissue, whereas carcinoma cells consistently showed reaction. Moreover, a statistically significant difference was seen between the level of reaction in carcinoma cells and that of ductal epithelial cells in fibrocystic disease not containing apocrine metaplasia. Apocrine metaplasia, on the other hand, showed the highest activity recorded at all.

AB - We here report a method for the characterization of carcinoma cells in cryosections of human breast tissue. Cryosections from 37 biopsies including carcinomas, fibrocystic disease specimens and reduction mammoplasties were incubated in an atmosphere of either pure nitrogen or 99.5% oxygen. The incubation medium contained neotetrazolium, phenazine methosulphate and the colloid stabilizer Polypep 5115. A marked overlap in formation of reaction product was found between carcinomas, benign tissue and normal breast tissue when sections were incubated in pure nitrogen. However, following 5 min of oxygen incubation no reaction was seen in normal breast tissue, whereas carcinoma cells consistently showed reaction. Moreover, a statistically significant difference was seen between the level of reaction in carcinoma cells and that of ductal epithelial cells in fibrocystic disease not containing apocrine metaplasia. Apocrine metaplasia, on the other hand, showed the highest activity recorded at all.

KW - Glucose 6-phosphate dehydrogenase cytochemistry

KW - Human breast tissue

KW - Oxygen effect on tetrazolium reaction

UR - http://www.scopus.com/inward/record.url?scp=0022414192&partnerID=8YFLogxK

U2 - 10.1007/BF02889887

DO - 10.1007/BF02889887

M3 - Journal article

C2 - 2867640

AN - SCOPUS:0022414192

VL - 50

SP - 13

EP - 25

JO - Virchows Archiv B Cell Pathology Including Molecular Pathology

JF - Virchows Archiv B Cell Pathology Including Molecular Pathology

SN - 0042-6431

IS - 1

ER -

ID: 347536505