Echinostoma caproni in mice: shedding of antigens from the surface of an intestinal trematode

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Standard

Echinostoma caproni in mice: shedding of antigens from the surface of an intestinal trematode. / Andresen, K; Simonsen, Paul Erik; Andersen, B J; Birch-Andersen, A.

I: International Journal for Parasitology, Bind 19, Nr. 1, 01.02.1989, s. 111-8.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Andresen, K, Simonsen, PE, Andersen, BJ & Birch-Andersen, A 1989, 'Echinostoma caproni in mice: shedding of antigens from the surface of an intestinal trematode', International Journal for Parasitology, bind 19, nr. 1, s. 111-8.

APA

Andresen, K., Simonsen, P. E., Andersen, B. J., & Birch-Andersen, A. (1989). Echinostoma caproni in mice: shedding of antigens from the surface of an intestinal trematode. International Journal for Parasitology, 19(1), 111-8.

Vancouver

Andresen K, Simonsen PE, Andersen BJ, Birch-Andersen A. Echinostoma caproni in mice: shedding of antigens from the surface of an intestinal trematode. International Journal for Parasitology. 1989 feb. 1;19(1):111-8.

Author

Andresen, K ; Simonsen, Paul Erik ; Andersen, B J ; Birch-Andersen, A. / Echinostoma caproni in mice: shedding of antigens from the surface of an intestinal trematode. I: International Journal for Parasitology. 1989 ; Bind 19, Nr. 1. s. 111-8.

Bibtex

@article{b10d35645ce34443b49e9d835b493b17,
title = "Echinostoma caproni in mice: shedding of antigens from the surface of an intestinal trematode",
abstract = "The surface antigens, which induce a serum antibody response during infection of mice with the intestinal trematode Echinostoma caproni, were examined. It was demonstrated that antigens are shed from the surface of juvenile and 4-week old adult E. caproni during in vitro culture. SDS-PAGE and Western blot analysis of in vitro shed and detergent solubilized surface antigens indicated that the four major antigens released from the surface of adult parasites had molecular masses of approximately 26,000, 66,000, 75,000 and 88,000. A modified ELISA technique showed the in vitro turn-over rate of the surface antigens to be very high, with a half-life of 8-15 min in both juvenile and adult E. caproni trematodes. Transmission electron microscopy of the surface of adult parasites revealed a highly active secreting tegument which was densely packed with membrane-bound vesicles, reflecting the high rate of shedding of the surface antigens. An attempt to immunize mice with detergent solubilized adult surface antigens failed to induce resistance to infection with metacercariae of E. caproni.",
author = "K Andresen and Simonsen, {Paul Erik} and Andersen, {B J} and A Birch-Andersen",
year = "1989",
month = feb,
day = "1",
language = "English",
volume = "19",
pages = "111--8",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Echinostoma caproni in mice: shedding of antigens from the surface of an intestinal trematode

AU - Andresen, K

AU - Simonsen, Paul Erik

AU - Andersen, B J

AU - Birch-Andersen, A

PY - 1989/2/1

Y1 - 1989/2/1

N2 - The surface antigens, which induce a serum antibody response during infection of mice with the intestinal trematode Echinostoma caproni, were examined. It was demonstrated that antigens are shed from the surface of juvenile and 4-week old adult E. caproni during in vitro culture. SDS-PAGE and Western blot analysis of in vitro shed and detergent solubilized surface antigens indicated that the four major antigens released from the surface of adult parasites had molecular masses of approximately 26,000, 66,000, 75,000 and 88,000. A modified ELISA technique showed the in vitro turn-over rate of the surface antigens to be very high, with a half-life of 8-15 min in both juvenile and adult E. caproni trematodes. Transmission electron microscopy of the surface of adult parasites revealed a highly active secreting tegument which was densely packed with membrane-bound vesicles, reflecting the high rate of shedding of the surface antigens. An attempt to immunize mice with detergent solubilized adult surface antigens failed to induce resistance to infection with metacercariae of E. caproni.

AB - The surface antigens, which induce a serum antibody response during infection of mice with the intestinal trematode Echinostoma caproni, were examined. It was demonstrated that antigens are shed from the surface of juvenile and 4-week old adult E. caproni during in vitro culture. SDS-PAGE and Western blot analysis of in vitro shed and detergent solubilized surface antigens indicated that the four major antigens released from the surface of adult parasites had molecular masses of approximately 26,000, 66,000, 75,000 and 88,000. A modified ELISA technique showed the in vitro turn-over rate of the surface antigens to be very high, with a half-life of 8-15 min in both juvenile and adult E. caproni trematodes. Transmission electron microscopy of the surface of adult parasites revealed a highly active secreting tegument which was densely packed with membrane-bound vesicles, reflecting the high rate of shedding of the surface antigens. An attempt to immunize mice with detergent solubilized adult surface antigens failed to induce resistance to infection with metacercariae of E. caproni.

M3 - Journal article

C2 - 2707956

VL - 19

SP - 111

EP - 118

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 1

ER -

ID: 32355893