TY - JOUR

T1 - Computer-assisted image analysis assay of human neutrophil chemotaxis in vitro

AU - Jensen, P

AU - Kharazmi, A

N1 - Keywords: Chemotaxis, Leukocyte; Humans; Image Processing, Computer-Assisted; N-Formylmethionine Leucyl-Phenylalanine; Neutrophils; Zymosan

PY - 1991

Y1 - 1991

N2 - We have developed a computer-based image analysis system to measure in-filter migration of human neutrophils in the Boyden chamber. This method is compared with the conventional manual counting techniques. Neutrophils from healthy individuals and from patients with reduced chemotactic activity were used. The cells migrating into the filter were counted automatically at depths 20 microns apart commencing from the upper surface on the filter. The major advantages of this method are reproducibility and the counting of many fields which provides better data for statistical analysis of the results. Another advantage of the assay is that it can be used to show the migration pattern of different populations of neutrophils from both healthy individuals and patients.

AB - We have developed a computer-based image analysis system to measure in-filter migration of human neutrophils in the Boyden chamber. This method is compared with the conventional manual counting techniques. Neutrophils from healthy individuals and from patients with reduced chemotactic activity were used. The cells migrating into the filter were counted automatically at depths 20 microns apart commencing from the upper surface on the filter. The major advantages of this method are reproducibility and the counting of many fields which provides better data for statistical analysis of the results. Another advantage of the assay is that it can be used to show the migration pattern of different populations of neutrophils from both healthy individuals and patients.

M3 - Journal article

C2 - 1960405

VL - 144

SP - 43

EP - 48

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

IS - 1

ER -