Colour development of immunogold-labelled antibodies for light microscopy.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Standard

Colour development of immunogold-labelled antibodies for light microscopy. / Fritz, P; Hoenes, J; Schenk, J; Mischlinski, A; Grau, A; Saal, J G; Tuczek, H V; Multhaupt, H; Pfleiderer, G.

I: Histochemistry, Bind 85, Nr. 3, 1986, s. 209-14.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Fritz, P, Hoenes, J, Schenk, J, Mischlinski, A, Grau, A, Saal, JG, Tuczek, HV, Multhaupt, H & Pfleiderer, G 1986, 'Colour development of immunogold-labelled antibodies for light microscopy.', Histochemistry, bind 85, nr. 3, s. 209-14.

APA

Fritz, P., Hoenes, J., Schenk, J., Mischlinski, A., Grau, A., Saal, J. G., Tuczek, H. V., Multhaupt, H., & Pfleiderer, G. (1986). Colour development of immunogold-labelled antibodies for light microscopy. Histochemistry, 85(3), 209-14.

Vancouver

Fritz P, Hoenes J, Schenk J, Mischlinski A, Grau A, Saal JG o.a. Colour development of immunogold-labelled antibodies for light microscopy. Histochemistry. 1986;85(3):209-14.

Author

Fritz, P ; Hoenes, J ; Schenk, J ; Mischlinski, A ; Grau, A ; Saal, J G ; Tuczek, H V ; Multhaupt, H ; Pfleiderer, G. / Colour development of immunogold-labelled antibodies for light microscopy. I: Histochemistry. 1986 ; Bind 85, Nr. 3. s. 209-14.

Bibtex

@article{4f8812e05d4411dd8d9f000ea68e967b,
title = "Colour development of immunogold-labelled antibodies for light microscopy.",
abstract = "We describe an improvement of the immunogold technique, which is based on the colour development of silver-intensified immunogold signals. This method (referred to as the coloured-SIG technique) was found to be as sensitive as the silver-intensified immunogold method and more sensitive (in two of the three tested systems) than immunoenzymatic procedures, such as the peroxidase/antiperoxidase technique or the avidin-biotin system. The coloured SIG-method results in either a magenta-red or a cyan-blue final reaction product. Therefore, this new improvement of the immunogold technique should be useful in double-staining methods, immunoblot methods and conventional immunostaining methods.",
author = "P Fritz and J Hoenes and J Schenk and A Mischlinski and A Grau and Saal, {J G} and Tuczek, {H V} and H Multhaupt and G Pfleiderer",
note = "Keywords: Antibodies; Antigens, Surface; Arthritis, Rheumatoid; Gold; Humans; Immunoenzyme Techniques; Microscopy; Osteoarthritis; Synovial Membrane; T-Lymphocytes; Tonsil",
year = "1986",
language = "English",
volume = "85",
pages = "209--14",
journal = "Histochemistry",
issn = "0301-5564",
publisher = "Springer",
number = "3",

}

RIS

TY - JOUR

T1 - Colour development of immunogold-labelled antibodies for light microscopy.

AU - Fritz, P

AU - Hoenes, J

AU - Schenk, J

AU - Mischlinski, A

AU - Grau, A

AU - Saal, J G

AU - Tuczek, H V

AU - Multhaupt, H

AU - Pfleiderer, G

N1 - Keywords: Antibodies; Antigens, Surface; Arthritis, Rheumatoid; Gold; Humans; Immunoenzyme Techniques; Microscopy; Osteoarthritis; Synovial Membrane; T-Lymphocytes; Tonsil

PY - 1986

Y1 - 1986

N2 - We describe an improvement of the immunogold technique, which is based on the colour development of silver-intensified immunogold signals. This method (referred to as the coloured-SIG technique) was found to be as sensitive as the silver-intensified immunogold method and more sensitive (in two of the three tested systems) than immunoenzymatic procedures, such as the peroxidase/antiperoxidase technique or the avidin-biotin system. The coloured SIG-method results in either a magenta-red or a cyan-blue final reaction product. Therefore, this new improvement of the immunogold technique should be useful in double-staining methods, immunoblot methods and conventional immunostaining methods.

AB - We describe an improvement of the immunogold technique, which is based on the colour development of silver-intensified immunogold signals. This method (referred to as the coloured-SIG technique) was found to be as sensitive as the silver-intensified immunogold method and more sensitive (in two of the three tested systems) than immunoenzymatic procedures, such as the peroxidase/antiperoxidase technique or the avidin-biotin system. The coloured SIG-method results in either a magenta-red or a cyan-blue final reaction product. Therefore, this new improvement of the immunogold technique should be useful in double-staining methods, immunoblot methods and conventional immunostaining methods.

M3 - Journal article

C2 - 3528080

VL - 85

SP - 209

EP - 214

JO - Histochemistry

JF - Histochemistry

SN - 0301-5564

IS - 3

ER -

ID: 5240608