Clinical evidence supporting the radical scavenger mechanism of 5-aminosalicylic acid
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Clinical evidence supporting the radical scavenger mechanism of 5-aminosalicylic acid. / Ahnfelt-Rønne, I; Nielsen, O H; Christensen, A; Langholz, E; Binder, V; Riis, P.
I: Gastroenterology, Bind 98, Nr. 5 Pt 1, 05.1990, s. 1162-9.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Clinical evidence supporting the radical scavenger mechanism of 5-aminosalicylic acid
AU - Ahnfelt-Rønne, I
AU - Nielsen, O H
AU - Christensen, A
AU - Langholz, E
AU - Binder, V
AU - Riis, P
PY - 1990/5
Y1 - 1990/5
N2 - 5-Aminosalicylic acid, the therapeutically active metabolite of sulfasalazine, was exposed to oxygen-derived free radicals produced by the Fenton reaction in vitro, and several metabolites were detected and characterized by high performance liquid chromatography and ultraviolet spectrophotometry. The majority of these metabolites were present in methanolic extracts of feces samples from sulfasalazine-treated patients with inflammatory bowel disease but not in rheumatoid arthritis patients with normal bowel function. The presence of these metabolites, which have not been demonstrated in vivo before, provides evidence of an interaction between 5-aminosalicylic acid and oxygen-derived free radicals in sulfasalazine-treated patients with inflammatory bowel disease. Since the concentration of lipid peroxides, which is dependent on the release of oxygen-derived free radicals, was significantly increased in pretreatment rectal biopsies of the patients, and further was normalized concomitantly with a significant improvement in disease activity over the 5-wk treatment period, an important role of the radical scavenger mechanism of 5-aminosalicylic acid in sulfasalazine therapy of chronic inflammatory bowel disease is strongly suggested.
AB - 5-Aminosalicylic acid, the therapeutically active metabolite of sulfasalazine, was exposed to oxygen-derived free radicals produced by the Fenton reaction in vitro, and several metabolites were detected and characterized by high performance liquid chromatography and ultraviolet spectrophotometry. The majority of these metabolites were present in methanolic extracts of feces samples from sulfasalazine-treated patients with inflammatory bowel disease but not in rheumatoid arthritis patients with normal bowel function. The presence of these metabolites, which have not been demonstrated in vivo before, provides evidence of an interaction between 5-aminosalicylic acid and oxygen-derived free radicals in sulfasalazine-treated patients with inflammatory bowel disease. Since the concentration of lipid peroxides, which is dependent on the release of oxygen-derived free radicals, was significantly increased in pretreatment rectal biopsies of the patients, and further was normalized concomitantly with a significant improvement in disease activity over the 5-wk treatment period, an important role of the radical scavenger mechanism of 5-aminosalicylic acid in sulfasalazine therapy of chronic inflammatory bowel disease is strongly suggested.
KW - Adult
KW - Aged
KW - Aminosalicylic Acids/analysis
KW - Biopsy
KW - Colitis, Ulcerative/drug therapy
KW - Colonic Diseases, Functional/drug therapy
KW - Crohn Disease/drug therapy
KW - Feces/analysis
KW - Female
KW - Free Radicals
KW - Humans
KW - Lipid Peroxidation/drug effects
KW - Male
KW - Mesalamine
KW - Middle Aged
KW - Oxidation-Reduction/drug effects
KW - Rectum/metabolism
KW - Sulfasalazine/analysis
KW - Time Factors
M3 - Journal article
C2 - 1969825
VL - 98
SP - 1162
EP - 1169
JO - Gastroenterology
JF - Gastroenterology
SN - 0016-5085
IS - 5 Pt 1
ER -
ID: 218728207