TY - JOUR

T1 - Chromatographic separation of alkaline phosphatase from dental enamel

AU - Moe, D

AU - Kirkeby, S

AU - Salling, E

N1 - Keywords: Alkaline Phosphatase; Animals; Cattle; Chromatography; Chromatography, Gel; Chromatography, High Pressure Liquid; Dental Enamel; Dental Enamel Proteins; Electrophoresis; Isoelectric Focusing; Spectrophotometry

PY - 1989

Y1 - 1989

N2 - Alkaline phosphatase (AP) was prepared from partly mineralized bovine enamel by extraction in phosphate buffer, centrifugation and various chromatographic techniques. Chromatofocusing showed that the enamel enzyme possessed five isoelectric points at the acid pH level ranging from pH 5.7 to pH 4.4. Three enzyme peaks were eluted using low pressure chromatography with a Bio-gel column. With a HPLC gel filtration column the separation of the enamel extract resulted in only one peak with AP activity. The fractions of this peak were used to produce an antibody against bovine AP.

AB - Alkaline phosphatase (AP) was prepared from partly mineralized bovine enamel by extraction in phosphate buffer, centrifugation and various chromatographic techniques. Chromatofocusing showed that the enamel enzyme possessed five isoelectric points at the acid pH level ranging from pH 5.7 to pH 4.4. Three enzyme peaks were eluted using low pressure chromatography with a Bio-gel column. With a HPLC gel filtration column the separation of the enamel extract resulted in only one peak with AP activity. The fractions of this peak were used to produce an antibody against bovine AP.

M3 - Journal article

C2 - 2711125

VL - 97

SP - 8

EP - 13

JO - Scandinavian Journal of Dental Research

JF - Scandinavian Journal of Dental Research

SN - 0029-845X

IS - 1

ER -