Characterizing core microbiota and regulatory functions of the pig gut microbiome

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningfagfællebedømt

Dokumenter

  • Fulltext

    Forlagets udgivne version, 5 MB, PDF-dokument

  • Jun Hu
  • Jianwei Chen
  • Libao Ma
  • Qiliang Hou
  • Zhang, Yong
  • Xiangfeng Kong
  • Xingguo Huang
  • Zhonglin Tang
  • Hong Wei
  • Xiangru Wang
  • Xianghua Yan
Domestic pigs (Sus scrofa) are the leading terrestrial animals used for meat production. The gut microbiota significantly affect host nutrition, metabolism, and immunity. Hence, characterization of the gut microbial structure and function will improve our understanding of gut microbial resources and the mechanisms underlying host–microbe interactions. Here, we investigated the gut microbiomes of seven pig breeds using metagenomics and 16S rRNA gene amplicon sequencing. We established an expanded gut microbial reference catalog comprising 17 020 160 genes and identified 4910 metagenome-assembled genomes. We also analyzed the gut resistome to provide an overview of the profiles of the antimicrobial resistance genes in pigs. By analyzing the relative abundances of microbes, we identified three core-predominant gut microbes (Phascolarctobacterium succinatutens, Prevotella copri, and Oscillibacter valericigenes) in pigs used in this study. Oral administration of the three core-predominant gut microbes significantly increased the organ indexes (including the heart, spleen, and thymus), but decreased the gastrointestinal lengths in germ-free mice. The three core microbes significantly enhanced intestinal epithelial barrier function and altered the intestinal mucosal morphology, as was evident from the increase in crypt depths in the duodenum and ileum. Furthermore, the three core microbes significantly affected several metabolic pathways (such as “steroid hormone biosynthesis,” “primary bile acid biosynthesis,” “phenylalanine, tyrosine and tryptophan biosynthesis,” and “phenylalanine metabolism”) in germ-free mice. These findings provide a panoramic view of the pig gut microbiome and insights into the functional contributions of the core-predominant gut microbes to the host.
OriginalsprogEngelsk
Artikelnummerwrad037
TidsskriftThe ISME Journal
Vol/bind18
Udgave nummer1
Antal sider17
ISSN1751-7362
DOI
StatusUdgivet - 2024
Eksternt udgivetJa

Bibliografisk note

Publisher Copyright:
© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.

ID: 384493535