Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization.

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Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization. / Multhaupt, H; Gross, G; Fritz, P; Köhler, K.

I: Histochemistry, Bind 91, Nr. 4, 1989, s. 315-9.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Multhaupt, H, Gross, G, Fritz, P & Köhler, K 1989, 'Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization.', Histochemistry, bind 91, nr. 4, s. 315-9.

APA

Multhaupt, H., Gross, G., Fritz, P., & Köhler, K. (1989). Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization. Histochemistry, 91(4), 315-9.

Vancouver

Multhaupt H, Gross G, Fritz P, Köhler K. Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization. Histochemistry. 1989;91(4):315-9.

Author

Multhaupt, H ; Gross, G ; Fritz, P ; Köhler, K. / Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization. I: Histochemistry. 1989 ; Bind 91, Nr. 4. s. 315-9.

Bibtex

@article{68285a905d4311dd8d9f000ea68e967b,
title = "Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization.",
abstract = "Induced interferon-beta (IFN-beta) mRNA was localized in human FS-4 fibroblasts by in situ hybridization using biotinylated probes. The hybridization sites were detected by incubation with a nick-translated genomic DNA probe (1.8 kb) via streptavidin-colloidal gold followed by silver contrast enhancement. The positive signals were observed by reflection-contrast light microscopy. IFN-beta mRNA was transiently induced by poly r(I): r(C) in fibroblasts 2-4 h after induction. Induction in the presence of cycloheximide and actinomycin D (superinduction conditions) exhibited an enhanced level of IFN-beta mRNA with a maximum at 4-8 h. The kinetics of the IFN-beta mRNA expression in the cytoplasm as revealed by in situ hybridization proved to be compatible with the results of Northern blotting experiments of total cellular RNA.",
author = "H Multhaupt and G Gross and P Fritz and K K{\"o}hler",
note = "Keywords: Cells, Cultured; Fibroblasts; Humans; Interferon Type I; Kinetics; Nucleic Acid Hybridization; RNA, Messenger",
year = "1989",
language = "English",
volume = "91",
pages = "315--9",
journal = "Histochemistry",
issn = "0301-5564",
publisher = "Springer",
number = "4",

}

RIS

TY - JOUR

T1 - Cellular localization of induced human interferon-beta mRNA by non-radioactive in situ hybridization.

AU - Multhaupt, H

AU - Gross, G

AU - Fritz, P

AU - Köhler, K

N1 - Keywords: Cells, Cultured; Fibroblasts; Humans; Interferon Type I; Kinetics; Nucleic Acid Hybridization; RNA, Messenger

PY - 1989

Y1 - 1989

N2 - Induced interferon-beta (IFN-beta) mRNA was localized in human FS-4 fibroblasts by in situ hybridization using biotinylated probes. The hybridization sites were detected by incubation with a nick-translated genomic DNA probe (1.8 kb) via streptavidin-colloidal gold followed by silver contrast enhancement. The positive signals were observed by reflection-contrast light microscopy. IFN-beta mRNA was transiently induced by poly r(I): r(C) in fibroblasts 2-4 h after induction. Induction in the presence of cycloheximide and actinomycin D (superinduction conditions) exhibited an enhanced level of IFN-beta mRNA with a maximum at 4-8 h. The kinetics of the IFN-beta mRNA expression in the cytoplasm as revealed by in situ hybridization proved to be compatible with the results of Northern blotting experiments of total cellular RNA.

AB - Induced interferon-beta (IFN-beta) mRNA was localized in human FS-4 fibroblasts by in situ hybridization using biotinylated probes. The hybridization sites were detected by incubation with a nick-translated genomic DNA probe (1.8 kb) via streptavidin-colloidal gold followed by silver contrast enhancement. The positive signals were observed by reflection-contrast light microscopy. IFN-beta mRNA was transiently induced by poly r(I): r(C) in fibroblasts 2-4 h after induction. Induction in the presence of cycloheximide and actinomycin D (superinduction conditions) exhibited an enhanced level of IFN-beta mRNA with a maximum at 4-8 h. The kinetics of the IFN-beta mRNA expression in the cytoplasm as revealed by in situ hybridization proved to be compatible with the results of Northern blotting experiments of total cellular RNA.

M3 - Journal article

C2 - 2732096

VL - 91

SP - 315

EP - 319

JO - Histochemistry

JF - Histochemistry

SN - 0301-5564

IS - 4

ER -

ID: 5240506