Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants

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Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants. / Cowell, G M; Danielsen, E M.

I: FEBS Letters, Bind 172, Nr. 2, 1984, s. 309-14.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Cowell, GM & Danielsen, EM 1984, 'Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants', FEBS Letters, bind 172, nr. 2, s. 309-14.

APA

Cowell, G. M., & Danielsen, E. M. (1984). Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants. FEBS Letters, 172(2), 309-14.

Vancouver

Cowell GM, Danielsen EM. Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants. FEBS Letters. 1984;172(2):309-14.

Author

Cowell, G M ; Danielsen, E M. / Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants. I: FEBS Letters. 1984 ; Bind 172, Nr. 2. s. 309-14.

Bibtex

@article{cdcc8c80e7be11ddbf70000ea68e967b,
title = "Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants",
abstract = "Using alkaline extraction to separate cytoskeletal and membrane proteins of intestinal microvilli, the kinetics of assembly of these two microvillar protein compartments was studied by pulse-chase labelling of pig small intestinal mucosal explants, kept in organ culture. Following a 10 min pulse of [35S]methionine, the membrane proteins did not appear in the microvillar fraction until after 40-60 min of chase. In contrast, the cytoskeletal components, of which the 110-kDa protein and villin were immunologically identified, were expressed in the microvillar fraction immediately after the 10 min pulse. These different kinetics of appearance indicate that the two microvillar protein compartments have separate mechanisms of biosynthesis and microvillar expression.",
author = "Cowell, {G M} and Danielsen, {E M}",
note = "Keywords: Animals; Cell Membrane; Cytoskeleton; Intestine, Small; Kinetics; Membrane Proteins; Microvilli; Molecular Weight; Organ Culture Techniques; Protein Biosynthesis; Proteins; Swine",
year = "1984",
language = "English",
volume = "172",
pages = "309--14",
journal = "F E B S Letters",
issn = "0014-5793",
publisher = "JohnWiley & Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Biosynthesis of intestinal microvillar proteins. Rapid expression of cytoskeletal components in microvilli of pig small intestinal mucosal explants

AU - Cowell, G M

AU - Danielsen, E M

N1 - Keywords: Animals; Cell Membrane; Cytoskeleton; Intestine, Small; Kinetics; Membrane Proteins; Microvilli; Molecular Weight; Organ Culture Techniques; Protein Biosynthesis; Proteins; Swine

PY - 1984

Y1 - 1984

N2 - Using alkaline extraction to separate cytoskeletal and membrane proteins of intestinal microvilli, the kinetics of assembly of these two microvillar protein compartments was studied by pulse-chase labelling of pig small intestinal mucosal explants, kept in organ culture. Following a 10 min pulse of [35S]methionine, the membrane proteins did not appear in the microvillar fraction until after 40-60 min of chase. In contrast, the cytoskeletal components, of which the 110-kDa protein and villin were immunologically identified, were expressed in the microvillar fraction immediately after the 10 min pulse. These different kinetics of appearance indicate that the two microvillar protein compartments have separate mechanisms of biosynthesis and microvillar expression.

AB - Using alkaline extraction to separate cytoskeletal and membrane proteins of intestinal microvilli, the kinetics of assembly of these two microvillar protein compartments was studied by pulse-chase labelling of pig small intestinal mucosal explants, kept in organ culture. Following a 10 min pulse of [35S]methionine, the membrane proteins did not appear in the microvillar fraction until after 40-60 min of chase. In contrast, the cytoskeletal components, of which the 110-kDa protein and villin were immunologically identified, were expressed in the microvillar fraction immediately after the 10 min pulse. These different kinetics of appearance indicate that the two microvillar protein compartments have separate mechanisms of biosynthesis and microvillar expression.

M3 - Journal article

C2 - 6540204

VL - 172

SP - 309

EP - 314

JO - F E B S Letters

JF - F E B S Letters

SN - 0014-5793

IS - 2

ER -

ID: 9881441