Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport

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Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport. / Danielsen, E M; Hansen, Gert Helge; Cowell, G M.

I: European Journal of Cell Biology, Bind 49, Nr. 1, 1989, s. 123-7.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Danielsen, EM, Hansen, GH & Cowell, GM 1989, 'Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport', European Journal of Cell Biology, bind 49, nr. 1, s. 123-7.

APA

Danielsen, E. M., Hansen, G. H., & Cowell, G. M. (1989). Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport. European Journal of Cell Biology, 49(1), 123-7.

Vancouver

Danielsen EM, Hansen GH, Cowell GM. Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport. European Journal of Cell Biology. 1989;49(1):123-7.

Author

Danielsen, E M ; Hansen, Gert Helge ; Cowell, G M. / Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport. I: European Journal of Cell Biology. 1989 ; Bind 49, Nr. 1. s. 123-7.

Bibtex

@article{1c8bbfa0e31211ddb5fc000ea68e967b,
title = "Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport",
abstract = "The effect of culture at 20 degrees C on biosynthesis of microvillar enzymes was studied in pig small intestinal mucosal explants. At this temperature, aminopeptidase N (EC 3.4.11.2) and sucrase-isomaltase (EC 3.2.1.48-10) both accumulated intracellularly, predominantly in their transient, high mannose-glycosylated form characteristic of the newly synthesized enzymes prior to the molecular processing taking place in the Golgi complex. The general morphology of the enterocyte was unaffected by culture at low temperature except for the Golgi complex where the cisternae appeared condensed and surrounded by numerous vesicles of 50 to 55 nm. Both molecular processing and microvillar expression could be restored by shifting the temperature to 37 degrees C. Culture at low temperature did not induce any missorting of newly synthesized aminopeptidase N, but both molecular processing and microvillar expression only resumed at a slow rate after increasing the temperature, suggesting that reorganization of the Golgi complex is a time-requiring process.",
author = "Danielsen, {E M} and Hansen, {Gert Helge} and Cowell, {G M}",
note = "Keywords: Animals; Cells, Cultured; Cold Temperature; Intestinal Mucosa; Membrane Proteins; Microscopy, Electron; Microvilli; Organ Culture Techniques; Swine",
year = "1989",
language = "English",
volume = "49",
pages = "123--7",
journal = "Cytobiologie",
issn = "0724-5130",
publisher = "Elsevier GmbH - Urban und Fischer",
number = "1",

}

RIS

TY - JOUR

T1 - Biosynthesis of intestinal microvillar proteins. Low temperature arrests both processing and intracellular transport

AU - Danielsen, E M

AU - Hansen, Gert Helge

AU - Cowell, G M

N1 - Keywords: Animals; Cells, Cultured; Cold Temperature; Intestinal Mucosa; Membrane Proteins; Microscopy, Electron; Microvilli; Organ Culture Techniques; Swine

PY - 1989

Y1 - 1989

N2 - The effect of culture at 20 degrees C on biosynthesis of microvillar enzymes was studied in pig small intestinal mucosal explants. At this temperature, aminopeptidase N (EC 3.4.11.2) and sucrase-isomaltase (EC 3.2.1.48-10) both accumulated intracellularly, predominantly in their transient, high mannose-glycosylated form characteristic of the newly synthesized enzymes prior to the molecular processing taking place in the Golgi complex. The general morphology of the enterocyte was unaffected by culture at low temperature except for the Golgi complex where the cisternae appeared condensed and surrounded by numerous vesicles of 50 to 55 nm. Both molecular processing and microvillar expression could be restored by shifting the temperature to 37 degrees C. Culture at low temperature did not induce any missorting of newly synthesized aminopeptidase N, but both molecular processing and microvillar expression only resumed at a slow rate after increasing the temperature, suggesting that reorganization of the Golgi complex is a time-requiring process.

AB - The effect of culture at 20 degrees C on biosynthesis of microvillar enzymes was studied in pig small intestinal mucosal explants. At this temperature, aminopeptidase N (EC 3.4.11.2) and sucrase-isomaltase (EC 3.2.1.48-10) both accumulated intracellularly, predominantly in their transient, high mannose-glycosylated form characteristic of the newly synthesized enzymes prior to the molecular processing taking place in the Golgi complex. The general morphology of the enterocyte was unaffected by culture at low temperature except for the Golgi complex where the cisternae appeared condensed and surrounded by numerous vesicles of 50 to 55 nm. Both molecular processing and microvillar expression could be restored by shifting the temperature to 37 degrees C. Culture at low temperature did not induce any missorting of newly synthesized aminopeptidase N, but both molecular processing and microvillar expression only resumed at a slow rate after increasing the temperature, suggesting that reorganization of the Golgi complex is a time-requiring process.

M3 - Journal article

C2 - 2759096

VL - 49

SP - 123

EP - 127

JO - Cytobiologie

JF - Cytobiologie

SN - 0724-5130

IS - 1

ER -

ID: 9748508