Bilateral bronchoalveolar lavage cytology profiles in a warmblood horse population during a 1-year period
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Bilateral bronchoalveolar lavage cytology profiles in a warmblood horse population during a 1-year period. / Rasmussen, Nanna; Karlsen, Pernille; Otten, Nina D.; Fjeldborg, Julie; Hansen, Sanni.
I: Journal of Veterinary Internal Medicine, 2024.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Bilateral bronchoalveolar lavage cytology profiles in a warmblood horse population during a 1-year period
AU - Rasmussen, Nanna
AU - Karlsen, Pernille
AU - Otten, Nina D.
AU - Fjeldborg, Julie
AU - Hansen, Sanni
N1 - Publisher Copyright: © 2024 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
PY - 2024
Y1 - 2024
N2 - Background: Bronchoalveolar lavage (BAL) cytology results from 1 lung might not be representative of both lungs. Objectives: To determine whether the lung site sampled would influence the horse's BAL cytology profile, and if a pooled BAL sample would be superior with regard to BAL cytology diagnosis in a cohort of healthy and subclinical asthmatic warmblood horses. Animals: Fifty-nine horses in 2021 and 70 horses in 2022, the follow-up included 53 of the same in each year. Methods: A cross-sectional study with follow-up included BAL cytology samples from individual lungs and from pooled BAL samples. The BAL samples were enumerated and differential cell count were applied to categorize the horses as control or with airway inflammation (AI). Results: Bronchoalveolar lavage mast cell count was higher in left lung compared to right lung (2021; median 1.6 [range, 0.6-3.3] vs 1.2 [0.7-1.5] P =.009, 2022; median 3.1 [2.1-4.2] vs 2.4 [1.7-3.4], P <.001) and compared to pooled samples (2022; median 2.6 [1.7-3.7], P <.001). Between year 2021 and 2022, 17 of the horses had changes in BAL cytology from control to AI or vice versa. Conclusions and Clinical Importance: Pooled BAL sample was the least reliable for detecting AI, and was not representative of the overall lung condition.
AB - Background: Bronchoalveolar lavage (BAL) cytology results from 1 lung might not be representative of both lungs. Objectives: To determine whether the lung site sampled would influence the horse's BAL cytology profile, and if a pooled BAL sample would be superior with regard to BAL cytology diagnosis in a cohort of healthy and subclinical asthmatic warmblood horses. Animals: Fifty-nine horses in 2021 and 70 horses in 2022, the follow-up included 53 of the same in each year. Methods: A cross-sectional study with follow-up included BAL cytology samples from individual lungs and from pooled BAL samples. The BAL samples were enumerated and differential cell count were applied to categorize the horses as control or with airway inflammation (AI). Results: Bronchoalveolar lavage mast cell count was higher in left lung compared to right lung (2021; median 1.6 [range, 0.6-3.3] vs 1.2 [0.7-1.5] P =.009, 2022; median 3.1 [2.1-4.2] vs 2.4 [1.7-3.4], P <.001) and compared to pooled samples (2022; median 2.6 [1.7-3.7], P <.001). Between year 2021 and 2022, 17 of the horses had changes in BAL cytology from control to AI or vice versa. Conclusions and Clinical Importance: Pooled BAL sample was the least reliable for detecting AI, and was not representative of the overall lung condition.
KW - endoscopic examination
KW - equine
KW - lung site
KW - mild-moderate equine asthma
KW - severe equine asthma
U2 - 10.1111/jvim.17118
DO - 10.1111/jvim.17118
M3 - Journal article
C2 - 38780440
AN - SCOPUS:85193921883
JO - Journal of Veterinary Internal Medicine
JF - Journal of Veterinary Internal Medicine
SN - 0891-6640
ER -
ID: 394542060