Analysis of the stimulative effect of thapsigargin, a non‐TPA‐type tumour promoter, on arachidonic acid metabolism in rat peritoneal macrophages
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Analysis of the stimulative effect of thapsigargin, a non‐TPA‐type tumour promoter, on arachidonic acid metabolism in rat peritoneal macrophages. / Ohuchi, Kazuo; Sugawara, Tadaki; Watanabe, Masako; Hirasawa, Noriyasu; Tsurufuji, Susumu; Fujiki, Hirota; Christensen, S. Brøgger; Sugimura, Takashi.
I: British Journal of Pharmacology, Bind 94, Nr. 3, 07.1988, s. 917-923.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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T1 - Analysis of the stimulative effect of thapsigargin, a non‐TPA‐type tumour promoter, on arachidonic acid metabolism in rat peritoneal macrophages
AU - Ohuchi, Kazuo
AU - Sugawara, Tadaki
AU - Watanabe, Masako
AU - Hirasawa, Noriyasu
AU - Tsurufuji, Susumu
AU - Fujiki, Hirota
AU - Christensen, S. Brøgger
AU - Sugimura, Takashi
PY - 1988/7
Y1 - 1988/7
N2 - At concentrations above 10 ng ml−1, the tumour promoter thapsigargin stimulates the release of radioactivity from [3H]‐arachidonic acid‐labelled macrophages harvested from rat peritoneal cavity. The release of radioactivity from prelabelled macrophages was augmented more than additively when the cells were incubated in the medium containing both thapsigargin (10 ng ml−1) and other tumour promoters (10 ng ml−1), such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), teleocidin and aplysiatoxin. Thapsigargin required extracellular Ca2+ for the stimulation of arachidonic acid release, while TPA did not. Cytoplasmic free calcium level was increased by thapsigargin treatment but not by TPA treatment. An inhibitor of protein kinases, H‐7 inhibited the effect of TPA dose‐dependently, whereas H‐7 did not inhibit that of thapsigargin. These results suggest that thapsigargin stimulates arachidonic acid release by a mechanism different from that of TPA, viz by acting as a selective Ca2+ mobilizer, but not by activating protein kinase C as TPA does. 1988 British Pharmacological Society
AB - At concentrations above 10 ng ml−1, the tumour promoter thapsigargin stimulates the release of radioactivity from [3H]‐arachidonic acid‐labelled macrophages harvested from rat peritoneal cavity. The release of radioactivity from prelabelled macrophages was augmented more than additively when the cells were incubated in the medium containing both thapsigargin (10 ng ml−1) and other tumour promoters (10 ng ml−1), such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA), teleocidin and aplysiatoxin. Thapsigargin required extracellular Ca2+ for the stimulation of arachidonic acid release, while TPA did not. Cytoplasmic free calcium level was increased by thapsigargin treatment but not by TPA treatment. An inhibitor of protein kinases, H‐7 inhibited the effect of TPA dose‐dependently, whereas H‐7 did not inhibit that of thapsigargin. These results suggest that thapsigargin stimulates arachidonic acid release by a mechanism different from that of TPA, viz by acting as a selective Ca2+ mobilizer, but not by activating protein kinase C as TPA does. 1988 British Pharmacological Society
UR - http://www.scopus.com/inward/record.url?scp=0023904952&partnerID=8YFLogxK
U2 - 10.1111/j.1476-5381.1988.tb11604.x
DO - 10.1111/j.1476-5381.1988.tb11604.x
M3 - Journal article
C2 - 3140994
AN - SCOPUS:0023904952
VL - 94
SP - 917
EP - 923
JO - British Journal of Pharmacology
JF - British Journal of Pharmacology
SN - 0007-1188
IS - 3
ER -
ID: 232599158