Adaptation of intestinal hydrolases to starvation in rats: effect of thyroid function
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Adaptation of intestinal hydrolases to starvation in rats: effect of thyroid function. / Galluser, M; Belkhou, R; Freund, J N; Duluc, I; Torp, N; Danielsen, Erik Michael; Raul, F.
I: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology, Bind 161, Nr. 4, 1991, s. 357-61.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Adaptation of intestinal hydrolases to starvation in rats: effect of thyroid function
AU - Galluser, M
AU - Belkhou, R
AU - Freund, J N
AU - Duluc, I
AU - Torp, N
AU - Danielsen, Erik Michael
AU - Raul, F
N1 - Keywords: Adaptation, Physiological; Aminopeptidases; Animals; Hydrolases; Intestine, Small; Lactase; Male; Proteins; RNA, Messenger; Rats; Rats, Inbred Strains; Starvation; Sucrase; Thyroid Gland; Thyroidectomy; beta-Galactosidase
PY - 1991
Y1 - 1991
N2 - The effects of long-term starvation on the activities of sucrase, lactase, and aminopeptidase, and on their respective mRNA were determined in the small intestine of thyroidectomized and sham-operated adult rats. Thyroidectomy reduced the protein loss at the level of the intestinal brush border membranes during starvation. Prolonged fasting caused a significant decrease in sucrase activity, but thyroidectomy partly prevented this effect. However, the amount of the corresponding mRNA dropped during long term starvation without incidence of thyroidectomy. Lactase activity in the brush border membranes was increased by starvation, and thyroidectomy caused a further elevation of the enzyme activity. Simultaneously, lactase mRNA content rose only slightly compared to the enzyme activity. Aminopeptidase activity and mRNA content decreased during starvation and thyroidectomy did not prevent this process. These results indicate that intestinal hydrolases respond non-coordinately to long-term food deprivation. In addition, the thyroid status of the animals has a direct influence on the adaptation of several brush border hydrolases to starvation. This suggests that the drop in plasma thyroid hormones during fasting allows a better maintenance of protein content and of hydrolase activities in the brush border membranes of the small intestine. These adaptive processes seemed to be partly controlled at a post-transcriptional level.
AB - The effects of long-term starvation on the activities of sucrase, lactase, and aminopeptidase, and on their respective mRNA were determined in the small intestine of thyroidectomized and sham-operated adult rats. Thyroidectomy reduced the protein loss at the level of the intestinal brush border membranes during starvation. Prolonged fasting caused a significant decrease in sucrase activity, but thyroidectomy partly prevented this effect. However, the amount of the corresponding mRNA dropped during long term starvation without incidence of thyroidectomy. Lactase activity in the brush border membranes was increased by starvation, and thyroidectomy caused a further elevation of the enzyme activity. Simultaneously, lactase mRNA content rose only slightly compared to the enzyme activity. Aminopeptidase activity and mRNA content decreased during starvation and thyroidectomy did not prevent this process. These results indicate that intestinal hydrolases respond non-coordinately to long-term food deprivation. In addition, the thyroid status of the animals has a direct influence on the adaptation of several brush border hydrolases to starvation. This suggests that the drop in plasma thyroid hormones during fasting allows a better maintenance of protein content and of hydrolase activities in the brush border membranes of the small intestine. These adaptive processes seemed to be partly controlled at a post-transcriptional level.
M3 - Journal article
C2 - 1939743
VL - 161
SP - 357
EP - 361
JO - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
JF - Journal of Comparative Physiology B: Biochemical, Systemic, and Environmental Physiology
SN - 0174-1578
IS - 4
ER -
ID: 13064342