A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression

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A TIMP-1 splice variant transcript : possible role in regulation of TIMP-1 expression. / Øbro, Nina Friesgård; Lademann, Ulrik Axel; Birkenkamp-Demtroder, Karin; Holten-Andersen, Lars; Brunner, Nils; Offenberg, Hanne Kjær.

I: Cancer Letters, Bind 262, Nr. 1, 2008, s. 64-70.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Øbro, NF, Lademann, UA, Birkenkamp-Demtroder, K, Holten-Andersen, L, Brunner, N & Offenberg, HK 2008, 'A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression', Cancer Letters, bind 262, nr. 1, s. 64-70. https://doi.org/10.1016/j.canlet.2007.11.030

APA

Øbro, N. F., Lademann, U. A., Birkenkamp-Demtroder, K., Holten-Andersen, L., Brunner, N., & Offenberg, H. K. (2008). A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression. Cancer Letters, 262(1), 64-70. https://doi.org/10.1016/j.canlet.2007.11.030

Vancouver

Øbro NF, Lademann UA, Birkenkamp-Demtroder K, Holten-Andersen L, Brunner N, Offenberg HK. A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression. Cancer Letters. 2008;262(1):64-70. https://doi.org/10.1016/j.canlet.2007.11.030

Author

Øbro, Nina Friesgård ; Lademann, Ulrik Axel ; Birkenkamp-Demtroder, Karin ; Holten-Andersen, Lars ; Brunner, Nils ; Offenberg, Hanne Kjær. / A TIMP-1 splice variant transcript : possible role in regulation of TIMP-1 expression. I: Cancer Letters. 2008 ; Bind 262, Nr. 1. s. 64-70.

Bibtex

@article{9810dec0a1c311ddb6ae000ea68e967b,
title = "A TIMP-1 splice variant transcript: possible role in regulation of TIMP-1 expression",
abstract = "A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue. Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.",
author = "{\O}bro, {Nina Friesg{\aa}rd} and Lademann, {Ulrik Axel} and Karin Birkenkamp-Demtroder and Lars Holten-Andersen and Nils Brunner and Offenberg, {Hanne Kj{\ae}r}",
year = "2008",
doi = "10.1016/j.canlet.2007.11.030",
language = "English",
volume = "262",
pages = "64--70",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

RIS

TY - JOUR

T1 - A TIMP-1 splice variant transcript

T2 - possible role in regulation of TIMP-1 expression

AU - Øbro, Nina Friesgård

AU - Lademann, Ulrik Axel

AU - Birkenkamp-Demtroder, Karin

AU - Holten-Andersen, Lars

AU - Brunner, Nils

AU - Offenberg, Hanne Kjær

PY - 2008

Y1 - 2008

N2 - A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue. Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.

AB - A splice variant of tissue inhibitor of metalloproteinases-1 (TIMP-1) mRNA lacking exon 2 (TIMP-1-v2) has been identified in human cancer cells and in colorectal and breast cancer tumors. The purpose of this study was (1) to study the level of full length TIMP-1 and TIMP-1-v2 transcripts in colorectal tumors; (2) to investigate if TIMP-1-v2 is translated to protein. Full length TIMP-1 and TIMP-1-v2 mRNA levels were compared between colorectal tumors and normal mucosa by Q-PCR. Both full length TIMP-1 and TIMP-1-v2 transcripts were upregulated in tumor tissue. However, the level of TIMP-1-v2 relative to full length TIMP-1 was higher in normal compared to tumor tissue. Translation of TIMP-1-v2 to protein was analyzed in CHO cells. In this system, no TIMP-1-v2 protein was produced. Thus, the variant transcript seems to be an untranslated mRNA. These findings suggest that alternative splicing of TIMP-1 pre-mRNA to TIMP-1-v2 mRNA might be involved in regulating TIMP-1 expression.

U2 - 10.1016/j.canlet.2007.11.030

DO - 10.1016/j.canlet.2007.11.030

M3 - Journal article

C2 - 18180096

VL - 262

SP - 64

EP - 70

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -

ID: 8097980