A synchronized, large-scale field experiment using Arabidopsis thaliana reveals the significance of the UV-B photoreceptor UVR8 under natural conditions
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This study determines the functional role of the plant ultraviolet-B radiation (UV-B) photoreceptor, UV RESISTANCE LOCUS 8 (UVR8) under natural conditions using a large-scale ‘synchronized-genetic-perturbation-field-experiment’. Laboratory experiments have demonstrated a role for UVR8 in UV-B responses but do not reflect the complexity of outdoor conditions where ‘genotype × environment’ interactions can mask laboratory-observed responses. Arabidopsis thaliana knockout mutant, uvr8-7, and the corresponding Wassilewskija wild type, were sown outdoors on the same date at 21 locations across Europe, ranging from 39°N to 67°N latitude. Growth and climatic data were monitored until bolting. At the onset of bolting, rosette size, dry weight, and phenolics and glucosinolates were quantified. The uvr8-7 mutant developed a larger rosette and contained less kaempferol glycosides, quercetin glycosides and hydroxycinnamic acid derivatives than the wild type across all locations, demonstrating a role for UVR8 under field conditions. UV effects on rosette size and kaempferol glycoside content were UVR8 dependent, but independent of latitude. In contrast, differences between wild type and uvr8-7 in total quercetin glycosides, and the quercetin-to-kaempferol ratio decreased with increasing latitude, that is, a more variable UV response. Thus, the large-scale synchronized approach applied demonstrates a location-dependent functional role of UVR8 under natural conditions.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Plant Cell and Environment |
| ISSN | 0140-7791 |
| DOI | |
| Status | E-pub ahead of print - 2024 |
Bibliografisk note
Funding Information:
The authors thank Marlies Dolezal for initial statistical support. The following authors gratefully acknowledge financial support: Alenka Gaberscik: Ministry of Higher Education, Science and Innovation, Republic of Slovenia (programme \u201CBiology of plants\u201D P1\u20100212); \u00C5ke Strid: The Carl Trygger Foundation for Scientific Research, Sweden (grant #CTS21:1666), the Knowledge Foundation, Sweden (grant #20130164), the Swedish Research Council Formas, Sweden (grants #942\u20132015\u2013516) and the Faculty for Business, Science and Technology at \u00D6rebro University; Andr\u00E1s Viczi\u00E1n: the Hungarian Scientific Research Fund (OTKA, K\u2010138022 and K\u2010132633); Beatriz D\u00E1der and Alberto Ferreres: State Investigation Agency, Spain (projects AGL2010\u201022196\u2010C02\u201001 and FPI BES\u20102011\u2010045885); Dolors Verdaguer and Laura Llorens: Spanish Government (project CGL2014\u201055976\u2010R); Javier Mart\u00EDnez\u2010Abaigar and Encarnaci\u00F3n N\u00FA\u00F1ez\u2010Olivera: the Government of La Rioja (project \u201CAfianza\u201D 2023/05); Marcel A. K. Jansen: Science Foundation Ireland (grant number 16\u2010IA\u20104418); Marie\u2010Theres Hauser: the Austrian Science Fund (FWF) (project F3707); Riitta Julkunen\u2010Tiitto: Academy of Finland (project 267360). The European UV community was supported through COST action FA0906 (UV4growth).
Publisher Copyright:
© 2024 The Author(s). Plant, Cell & Environment published by John Wiley & Sons Ltd.
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