15-hydroxyprostaglandin dehydrogenase activity in vitro in lung and kidney of essential fatty acid-deficient rats
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Standard
15-hydroxyprostaglandin dehydrogenase activity in vitro in lung and kidney of essential fatty acid-deficient rats. / Hansen, Harald S.; Toft, B.S.
I: BBA General Subjects, Bind 529, Nr. 2, 01.01.1978, s. 230-236.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - 15-hydroxyprostaglandin dehydrogenase activity in vitro in lung and kidney of essential fatty acid-deficient rats
AU - Hansen, Harald S.
AU - Toft, B.S.
PY - 1978/1/1
Y1 - 1978/1/1
N2 - Weanling rats were fed for 6 months on a diet deficient in essential fatty acids: either fat-free, or with 28% (w/w) partially hydrogenated fish oil. Control rats were fed a diet with 28% (w/w) arachis oil for 6 months. 15-Hydroxyprostaglandin dehydrogenase activity was determined as initial rates of formation of H-labelled 15-keto-dihydro-prostaglandin E plus 15-keto-prostaglandin E in high speed supernatants of lung and kidney from each of the groups of rats. Dehydrogenase activity (expressed as either pmol/min per mg soluble protein, or as nmol/min per g tissue) was decreased 30-40% in the lungs of the two groups on diets deficient in essential fatty acids as compared to the control group. No difference was observed in dehydrogenase activity in the kidneys. The dehydrogenase may be of importance for the regulation of the level of endogenous prostaglandins and, thus, a decrease in activity could result in a slower turnover of prostaglandins.
AB - Weanling rats were fed for 6 months on a diet deficient in essential fatty acids: either fat-free, or with 28% (w/w) partially hydrogenated fish oil. Control rats were fed a diet with 28% (w/w) arachis oil for 6 months. 15-Hydroxyprostaglandin dehydrogenase activity was determined as initial rates of formation of H-labelled 15-keto-dihydro-prostaglandin E plus 15-keto-prostaglandin E in high speed supernatants of lung and kidney from each of the groups of rats. Dehydrogenase activity (expressed as either pmol/min per mg soluble protein, or as nmol/min per g tissue) was decreased 30-40% in the lungs of the two groups on diets deficient in essential fatty acids as compared to the control group. No difference was observed in dehydrogenase activity in the kidneys. The dehydrogenase may be of importance for the regulation of the level of endogenous prostaglandins and, thus, a decrease in activity could result in a slower turnover of prostaglandins.
UR - http://www.scopus.com/inward/record.url?scp=0018200354&partnerID=8YFLogxK
M3 - Journal article
AN - SCOPUS:0018200354
VL - 529
SP - 230
EP - 236
JO - B B A - General Subjects
JF - B B A - General Subjects
SN - 0304-4165
IS - 2
ER -
ID: 45561411