GLP-2 stimulates colonic growth via KGF, released by subepithelial myofibroblasts with GLP-2 receptors.
Research output: Contribution to journal › Journal article › Research › peer-review
BACKGROUND: Glucagon-like peptide-2 is thought to act as a growth factor for the gut, but the localization of the GLP-2 receptor and mechanism of action on epithelial growth is unclear. METHODS AND RESULTS: We found glucagon-like peptide-2 (GLP-2) receptors mainly on subepithelial myofibroblasts in rat, mouse, marmoset and human small and large intestine by immunohistochemistry and in situ hybridisation. By double labelling we found that these GLP-2 receptor immunoreactive cells also produce smooth muscle actin and keratinocyte growth factor (KGF). By subcutaneous infusion of either GLP-2 alone, GLP-2 plus KGF antibody, KGF antibody alone or saline in mice, we found that KGF antibody abolished the growth promoting effect of GLP-2 in the large intestine, but not in the small intestine. CONCLUSIONS: Our findings suggest that GLP-2 in the gut acts by activating receptors on the subepithelial myofibroblasts, causing the release of growth factors, which in turn stimulate intestinal growth.
Original language | English |
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Journal | Regulatory Peptides |
Volume | 124 |
Issue number | 1-3 |
Pages (from-to) | 105-12 |
Number of pages | 7 |
ISSN | 0167-0115 |
DOIs | |
Publication status | Published - 2005 |
Bibliographical note
Keywords: Actins; Animals; Cell Proliferation; Colon; Female; Fibroblast Growth Factor 7; Fibroblast Growth Factors; Fibroblasts; Glucagon-Like Peptide 2; Glucagon-Like Peptides; Humans; Mice; Peptides; Rats; Receptors, Glucagon
ID: 8418118