Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method

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Standard

Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method. / Sitsel, Oleg; Wang, Kaituo; Liu, Xiangyu; Gourdon, Pontus.

P-Type ATPases: Methods and Protocols. red. / Maike Bublitz. Bind 1377 Springer, 2016. s. 413-420 (Methods in Molecular Biology, Bind 1377).

Publikation: Bidrag til bog/antologi/rapport › Bidrag til bog/antologi › Forskning › fagfællebedømt

Harvard

Sitsel, O, Wang, K, Liu, X & Gourdon, P 2016, Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method. i M Bublitz (red.), P-Type ATPases: Methods and Protocols. bind 1377, Springer, Methods in Molecular Biology, bind 1377, s. 413-420. https://doi.org/10.1007/978-1-4939-3179-8_37

APA

Sitsel, O., Wang, K., Liu, X., & Gourdon, P. (2016). Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method. I M. Bublitz (red.), P-Type ATPases: Methods and Protocols (Bind 1377, s. 413-420). Springer. Methods in Molecular Biology Bind 1377 https://doi.org/10.1007/978-1-4939-3179-8_37

Vancouver

Sitsel O, Wang K, Liu X, Gourdon P. Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method. I Bublitz M, red., P-Type ATPases: Methods and Protocols. Bind 1377. Springer. 2016. s. 413-420. (Methods in Molecular Biology, Bind 1377). https://doi.org/10.1007/978-1-4939-3179-8_37

Author

Sitsel, Oleg ; Wang, Kaituo ; Liu, Xiangyu ; Gourdon, Pontus. / Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method. P-Type ATPases: Methods and Protocols. red. / Maike Bublitz. Bind 1377 Springer, 2016. s. 413-420 (Methods in Molecular Biology, Bind 1377).

Bibtex

@inbook{f744a65d86364065ab30ec48b439964d,
title = "Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method",
abstract = "Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ({"}HiLiDe{"}) circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.",
author = "Oleg Sitsel and Kaituo Wang and Xiangyu Liu and Pontus Gourdon",
year = "2016",
doi = "10.1007/978-1-4939-3179-8_37",
language = "English",
isbn = "978-1-4939-3178-1",
volume = "1377",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "413--420",
editor = "Maike Bublitz",
booktitle = "P-Type ATPases",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method

AU - Sitsel, Oleg

AU - Wang, Kaituo

AU - Liu, Xiangyu

AU - Gourdon, Pontus

PY - 2016

Y1 - 2016

N2 - Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.

AB - Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.

U2 - 10.1007/978-1-4939-3179-8_37

DO - 10.1007/978-1-4939-3179-8_37

M3 - Book chapter

C2 - 26695052

SN - 978-1-4939-3178-1

VL - 1377

T3 - Methods in Molecular Biology

SP - 413

EP - 420

BT - P-Type ATPases

A2 - Bublitz, Maike

PB - Springer

ER -

ID: 174864931