Crystallization of P-type ATPases by the High Lipid-Detergent (HiLiDe) Method

Publikation: Bidrag til bog/antologi/rapportBidrag til bog/antologiForskningfagfællebedømt

Determining structures of membrane proteins remains a significant challenge. A technique utilizing high lipid-detergent concentrations ("HiLiDe") circumvents the major bottlenecks of current membrane protein crystallization methods. During HiLiDe, the protein-lipid-detergent ratio is varied in a controlled way in order to yield initial crystal hits, which may be subsequently optimized by variation of the crystallization conditions and/or utilizing secondary detergents. HiLiDe preserves the advantages of classical lipid-based methods, yet is compatible with both the vapor diffusion and batch crystallization techniques. The method has been applied with particular success to P-type ATPases.

OriginalsprogEngelsk
TitelP-Type ATPases : Methods and Protocols
RedaktørerMaike Bublitz
Antal sider8
Vol/bind1377
ForlagSpringer
Publikationsdato2016
Sider413-420
Kapitel37
ISBN (Trykt)978-1-4939-3178-1
ISBN (Elektronisk)978-1-4939-3179-8
DOI
StatusUdgivet - 2016
NavnMethods in Molecular Biology
Vol/bind1377
ISSN1064-3745

ID: 174864931