Reduced circulating oxidized LDL is associated with hypocholesterolemia and enhanced thiol status in Gilbert syndrome

Research output: Contribution to journalJournal article

  • Ai-Ching Boon
  • Hawkins, Clare Louise
  • Kavita Bisht
  • Jeff S Coombes
  • Bhavisha Bakrania
  • Karl-Heinz Wagner
  • Andrew C Bulmer

A protective association between bilirubin and atherosclerosis/ischemic heart disease clearly exists in vivo. However, the relationship between bilirubin and in vivo oxidative stress parameters in a clinical population remains poorly described. The aim of this study was to assess whether persons expressing Gilbert syndrome (GS; i.e., unconjugated hyperbilirubinemia) are protected from thiol oxidation and to determine if this, in addition to their improved lipoprotein profile, could explain reduced oxidized low-density lipoprotein (oxLDL) status in them. Forty-four matched GS and control subjects were recruited and blood was prepared for the analysis of lipid profile and multiple plasma antioxidants and measures of oxidative stress. GS subjects possessed elevated plasma reduced thiol (8.03±1.09 versus 6.75±1.39 nmol/mg protein; P<0.01) and glutathione concentrations (12.7±2.39 versus 9.44±2.45 μM; P<0.001). Oxidative stress status (reduced:oxidized glutathione; GSH:GSSG) was significantly improved in GS (0.49±0.16 versus 0.32±0.12; P<0.001). and associated bilirubin carbonyl concentrations in lower negatively persons protein significantly were with>40 μM bilirubin versus controls (<17.1 μmol/L; P<0.05). Furthermore, absolute oxLDL concentrations were significantly lower in GS subjects (P<0.05). Forward stepwise regression analysis revealed that bilirubin was associated with increased GSH:GSSG ratio and reduced thiol concentrations, which, in addition to reduced circulating LDL, probably decreased oxLDL concentrations within the cohort. In addition, a marked reduction in total cholesterol concentrations in hyperbilirubinemic Gunn rats is presented (Gunn 0.57±0.09 versus control 1.69±0.40 mmol/L; P<0.001), arguing for a novel role for bilirubin in modulating lipid status in vivo. These findings implicate the physiological importance of bilirubin in protecting from atherosclerosis by reducing thiol and subsequent lipoprotein oxidation, in addition to reducing circulating LDL concentrations.

Original languageEnglish
JournalFree Radical Biology & Medicine
Volume52
Issue number10
Pages (from-to)2120-7
Number of pages8
ISSN0891-5849
DOIs
Publication statusPublished - 15 May 2012
Externally publishedYes

    Research areas

  • Adolescent, Adult, Animals, Antioxidants, Atherosclerosis, Bilirubin, Cholesterol, Cohort Studies, Female, Gilbert Disease, Glutathione, Humans, Lipids, Lipoproteins, LDL, Male, Middle Aged, Oxidative Stress, Rats, Sulfhydryl Compounds, Young Adult, Journal Article, Research Support, Non-U.S. Gov't

ID: 174497231