Pharmacokinetic analysis of [68Ga]Ga-DOTA-TOC PET in meningiomas for assessment of in vivo somatostatin receptor subtype 2
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Purpose DOTA-D-Phe(1)-Tyr(3)-octreotide with gallium-68 ([Ga-68]Ga-DOTA-TOC) is one of the PET tracers that forms the basis for peptide receptor radionuclide therapy based on somatostatin receptor subtype 2 (SSTR2) expression in meningiomas. Yet, the quantitative relationship between [Ga-68]Ga-DOTA-TOC accumulation and SSTR2 is unknown. We conducted a correlative analysis of a range of [Ga-68]Ga-DOTA-TOC PET metric(s) as imaging surrogate(s) of the receptor binding in meningiomas by correlating the PET results with SSTR2 expression from surgical specimens. We additionally investigated possible influences of secondary biological factors such as vascularization, inflammation and proliferation. Methods Fifteen patients with MRI-presumed or recurrent meningiomas underwent a 60-min dynamic [Ga-68]Ga-DOTA-TOC PET/CT before surgery. The PET data comprised maximum and mean standardized uptake values (SUVmax, SUVmean) with and without normalization to reference regions, and quantitative measurements derived from kinetic modelling using a reversible two-tissue compartment model with the fractional blood volume (V-B). Expressions of SSTR2 and proliferation (Ki-67, phosphohistone-H3, proliferating cell nuclear antigen) were determined by immunohistochemistry and/or quantitative polymerase chain reaction (qPCR), while biomarkers of vascularization (vascular endothelial growth factor A (VEGFA), endothelial marker CD34) and inflammation (cytokine interleukin-18, microglia/macrophage-specific marker CD68) by qPCR. Results Histopathology revealed 12 World Health Organization (WHO) grade I and three WHO grade II meningiomas showing no link to SSTR2. The majority of [Ga-68]Ga-DOTA-TOC PET metrics showed significant associations with SSTR2 protein, while all PET metrics were positively correlated with SSTR2 mRNA with the best results for mean tumour-to-blood ratio (TBRmean) (r = 0.757,P = 0.001) and SUVmean(r = 0.714,P = 0.003). Significant positive correlations were also found between [Ga-68]Ga-DOTA-TOC PET metrics, and VEGFA andV(B). SSTR2 mRNA was moderately correlated with VEGFA (r = 0.539,P = 0.038). Neither [Ga-68]Ga-DOTA-TOC PET metrics nor SSTR2 were correlated with proliferation or inflammation. Conclusion [Ga-68]Ga-DOTA-TOC accumulation in meningiomas is associated with SSTR2 binding and vascularization with TBR(mean)being the best PET metric for assessing SSTR2.
|Journal||European Journal of Nuclear Medicine and Molecular Imaging|
|Publication status||Published - 2020|
- Meningioma, [Ga-68]Ga-DOTA-TOC, SSTR2, VEGFA, ENDOTHELIAL GROWTH-FACTOR, PHASE-II TRIAL, GA-68-DOTATOC, THERAPY, EXPRESSION, TUMORS, Y-90-DOTATOC, PREDICTOR, RECURRENT, PATTERNS