Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner: implications for peroxynitrite-mediated damage at sites of inflammation
Research output: Contribution to journal › Journal article › Research › peer-review
Eleanor C Kennett, Michael Jonathan Davies
Glycosaminoglycans (long-chain polysaccharides) are major components of the extracellular matrix, glycocalyx, and synovial fluid. These materials provide strength and elasticity to tissues and play a key role in regulating cell behavior. Modifications to these materials have been linked to multiple human pathologies. Although modification may occur via both enzymatic and nonenzymatic mechanisms, there is considerable evidence for oxidant-mediated matrix damage. Peroxynitrite (ONOO(-)/ONOOH) is a potential mediator of such damage, as elevated levels of this oxidant are likely to be present at sites of inflammation. In this study we demonstrate that hyaluronan and chondroitin sulfate are extensively depolymerized by HO(.) and CO3(.-), but not NO2(.), which may be formed from peroxynitrite. Polymer fragmentation is shown to be dependent on the radical flux, to be O2-independent, and to occur in a site-selective manner as indicated by the detection of disaccharide fragments. EPR spin trapping experiments with polymers, oligomers, and component monosaccharides, including 13C-labeled materials, have provided evidence for the formation of specific carbon-centered sugar-derived radicals. The time course of formation of these radicals is consistent with these species being involved in polymer fragmentation.
|Journal||Free Radical Biology & Medicine|
|Number of pages||12|
|Publication status||Published - 15 Aug 2009|
- Carbon Radioisotopes, Carbonates, Chondroitin Sulfates, Electron Spin Resonance Spectroscopy, Extracellular Matrix, Free Radicals, Glycocalyx, Glycosaminoglycans, Humans, Hyaluronic Acid, Hydroxyl Radical, Inflammation, Nitrogen Dioxide, Oxidative Stress, Peroxynitrous Acid, Polymers, Synovial Fluid