Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner: implications for peroxynitrite-mediated damage at sites of inflammation

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Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner : implications for peroxynitrite-mediated damage at sites of inflammation. / Kennett, Eleanor C; Davies, Michael Jonathan.

In: Free Radical Biology & Medicine, Vol. 47, No. 4, 15.08.2009, p. 389-400.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Kennett, EC & Davies, MJ 2009, 'Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner: implications for peroxynitrite-mediated damage at sites of inflammation', Free Radical Biology & Medicine, vol. 47, no. 4, pp. 389-400. https://doi.org/10.1016/j.freeradbiomed.2009.05.002

APA

Kennett, E. C., & Davies, M. J. (2009). Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner: implications for peroxynitrite-mediated damage at sites of inflammation. Free Radical Biology & Medicine, 47(4), 389-400. https://doi.org/10.1016/j.freeradbiomed.2009.05.002

Vancouver

Kennett EC, Davies MJ. Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner: implications for peroxynitrite-mediated damage at sites of inflammation. Free Radical Biology & Medicine. 2009 Aug 15;47(4):389-400. https://doi.org/10.1016/j.freeradbiomed.2009.05.002

Author

Kennett, Eleanor C ; Davies, Michael Jonathan. / Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner : implications for peroxynitrite-mediated damage at sites of inflammation. In: Free Radical Biology & Medicine. 2009 ; Vol. 47, No. 4. pp. 389-400.

Bibtex

@article{485de056700042d19fc054f8f7a7a8ff,
title = "Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner: implications for peroxynitrite-mediated damage at sites of inflammation",
abstract = "Glycosaminoglycans (long-chain polysaccharides) are major components of the extracellular matrix, glycocalyx, and synovial fluid. These materials provide strength and elasticity to tissues and play a key role in regulating cell behavior. Modifications to these materials have been linked to multiple human pathologies. Although modification may occur via both enzymatic and nonenzymatic mechanisms, there is considerable evidence for oxidant-mediated matrix damage. Peroxynitrite (ONOO(-)/ONOOH) is a potential mediator of such damage, as elevated levels of this oxidant are likely to be present at sites of inflammation. In this study we demonstrate that hyaluronan and chondroitin sulfate are extensively depolymerized by HO(.) and CO3(.-), but not NO2(.), which may be formed from peroxynitrite. Polymer fragmentation is shown to be dependent on the radical flux, to be O2-independent, and to occur in a site-selective manner as indicated by the detection of disaccharide fragments. EPR spin trapping experiments with polymers, oligomers, and component monosaccharides, including 13C-labeled materials, have provided evidence for the formation of specific carbon-centered sugar-derived radicals. The time course of formation of these radicals is consistent with these species being involved in polymer fragmentation.",
keywords = "Carbon Radioisotopes, Carbonates, Chondroitin Sulfates, Electron Spin Resonance Spectroscopy, Extracellular Matrix, Free Radicals, Glycocalyx, Glycosaminoglycans, Humans, Hyaluronic Acid, Hydroxyl Radical, Inflammation, Nitrogen Dioxide, Oxidative Stress, Peroxynitrous Acid, Polymers, Synovial Fluid",
author = "Kennett, {Eleanor C} and Davies, {Michael Jonathan}",
year = "2009",
month = "8",
day = "15",
doi = "10.1016/j.freeradbiomed.2009.05.002",
language = "English",
volume = "47",
pages = "389--400",
journal = "Free Radical Biology & Medicine",
issn = "0891-5849",
publisher = "Elsevier",
number = "4",

}

RIS

TY - JOUR

T1 - Glycosaminoglycans are fragmented by hydroxyl, carbonate, and nitrogen dioxide radicals in a site-selective manner

T2 - implications for peroxynitrite-mediated damage at sites of inflammation

AU - Kennett, Eleanor C

AU - Davies, Michael Jonathan

PY - 2009/8/15

Y1 - 2009/8/15

N2 - Glycosaminoglycans (long-chain polysaccharides) are major components of the extracellular matrix, glycocalyx, and synovial fluid. These materials provide strength and elasticity to tissues and play a key role in regulating cell behavior. Modifications to these materials have been linked to multiple human pathologies. Although modification may occur via both enzymatic and nonenzymatic mechanisms, there is considerable evidence for oxidant-mediated matrix damage. Peroxynitrite (ONOO(-)/ONOOH) is a potential mediator of such damage, as elevated levels of this oxidant are likely to be present at sites of inflammation. In this study we demonstrate that hyaluronan and chondroitin sulfate are extensively depolymerized by HO(.) and CO3(.-), but not NO2(.), which may be formed from peroxynitrite. Polymer fragmentation is shown to be dependent on the radical flux, to be O2-independent, and to occur in a site-selective manner as indicated by the detection of disaccharide fragments. EPR spin trapping experiments with polymers, oligomers, and component monosaccharides, including 13C-labeled materials, have provided evidence for the formation of specific carbon-centered sugar-derived radicals. The time course of formation of these radicals is consistent with these species being involved in polymer fragmentation.

AB - Glycosaminoglycans (long-chain polysaccharides) are major components of the extracellular matrix, glycocalyx, and synovial fluid. These materials provide strength and elasticity to tissues and play a key role in regulating cell behavior. Modifications to these materials have been linked to multiple human pathologies. Although modification may occur via both enzymatic and nonenzymatic mechanisms, there is considerable evidence for oxidant-mediated matrix damage. Peroxynitrite (ONOO(-)/ONOOH) is a potential mediator of such damage, as elevated levels of this oxidant are likely to be present at sites of inflammation. In this study we demonstrate that hyaluronan and chondroitin sulfate are extensively depolymerized by HO(.) and CO3(.-), but not NO2(.), which may be formed from peroxynitrite. Polymer fragmentation is shown to be dependent on the radical flux, to be O2-independent, and to occur in a site-selective manner as indicated by the detection of disaccharide fragments. EPR spin trapping experiments with polymers, oligomers, and component monosaccharides, including 13C-labeled materials, have provided evidence for the formation of specific carbon-centered sugar-derived radicals. The time course of formation of these radicals is consistent with these species being involved in polymer fragmentation.

KW - Carbon Radioisotopes

KW - Carbonates

KW - Chondroitin Sulfates

KW - Electron Spin Resonance Spectroscopy

KW - Extracellular Matrix

KW - Free Radicals

KW - Glycocalyx

KW - Glycosaminoglycans

KW - Humans

KW - Hyaluronic Acid

KW - Hydroxyl Radical

KW - Inflammation

KW - Nitrogen Dioxide

KW - Oxidative Stress

KW - Peroxynitrous Acid

KW - Polymers

KW - Synovial Fluid

U2 - 10.1016/j.freeradbiomed.2009.05.002

DO - 10.1016/j.freeradbiomed.2009.05.002

M3 - Journal article

C2 - 19427378

VL - 47

SP - 389

EP - 400

JO - Free Radical Biology & Medicine

JF - Free Radical Biology & Medicine

SN - 0891-5849

IS - 4

ER -

ID: 129670395