Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients
Research output: Contribution to journal › Journal article › Research › peer-review
Serum protein oxidation levels in people with the autoimmune disease systemic lupus erythematosus (SLE) have previously been shown to (a) be elevated at a single time point and (b) correlate with disease activity. This study investigates whether this elevation is a chronic phenomenon, by analysis of multiple serum samples collected from 21 SLE patients and nine controls over a period of up to 38 months. Protein thiols were chronically decreased in SLE patients with stable or variable disease activity compared to controls, whilst protein-bound carbonyls and glycine were chronically increased. 2D-gel analysis of carbonyl distribution showed albumin and immunoglobulins to be particularly affected. In SLE patients with stable disease activity, higher long-term protein oxidation correlated with higher long-term disease activity. SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE.
|Free Radical Research
|Number of pages
|Published - Feb 2009
- Adult, Blood Proteins, Blotting, Western, Case-Control Studies, Cohort Studies, Disease Progression, Electrophoresis, Gel, Two-Dimensional, Female, Humans, Lupus Erythematosus, Systemic, Male, Middle Aged, Oxidation-Reduction, Oxidative Stress, Protein Binding, Severity of Illness Index