Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients
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Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients. / Morgan, Philip E; Sturgess, Allan D; Davies, Michael Jonathan.
In: Free Radical Research, Vol. 43, No. 2, 02.2009, p. 117-27.Research output: Contribution to journal › Journal article › peer-review
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TY - JOUR
T1 - Evidence for chronically elevated serum protein oxidation in systemic lupus erythematosus patients
AU - Morgan, Philip E
AU - Sturgess, Allan D
AU - Davies, Michael Jonathan
PY - 2009/2
Y1 - 2009/2
N2 - Serum protein oxidation levels in people with the autoimmune disease systemic lupus erythematosus (SLE) have previously been shown to (a) be elevated at a single time point and (b) correlate with disease activity. This study investigates whether this elevation is a chronic phenomenon, by analysis of multiple serum samples collected from 21 SLE patients and nine controls over a period of up to 38 months. Protein thiols were chronically decreased in SLE patients with stable or variable disease activity compared to controls, whilst protein-bound carbonyls and glycine were chronically increased. 2D-gel analysis of carbonyl distribution showed albumin and immunoglobulins to be particularly affected. In SLE patients with stable disease activity, higher long-term protein oxidation correlated with higher long-term disease activity. SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE.
AB - Serum protein oxidation levels in people with the autoimmune disease systemic lupus erythematosus (SLE) have previously been shown to (a) be elevated at a single time point and (b) correlate with disease activity. This study investigates whether this elevation is a chronic phenomenon, by analysis of multiple serum samples collected from 21 SLE patients and nine controls over a period of up to 38 months. Protein thiols were chronically decreased in SLE patients with stable or variable disease activity compared to controls, whilst protein-bound carbonyls and glycine were chronically increased. 2D-gel analysis of carbonyl distribution showed albumin and immunoglobulins to be particularly affected. In SLE patients with stable disease activity, higher long-term protein oxidation correlated with higher long-term disease activity. SLE patients with variable disease activity exhibited varying correlations between protein oxidation and disease activity markers. These results further support a role for oxidative stress in the pathogenesis of SLE.
KW - Adult
KW - Blood Proteins
KW - Blotting, Western
KW - Case-Control Studies
KW - Cohort Studies
KW - Disease Progression
KW - Electrophoresis, Gel, Two-Dimensional
KW - Female
KW - Humans
KW - Lupus Erythematosus, Systemic
KW - Male
KW - Middle Aged
KW - Oxidation-Reduction
KW - Oxidative Stress
KW - Protein Binding
KW - Severity of Illness Index
U2 - 10.1080/10715760802623896
DO - 10.1080/10715760802623896
M3 - Journal article
C2 - 19096973
VL - 43
SP - 117
EP - 127
JO - Free Radical Research
JF - Free Radical Research
SN - 1071-5762
IS - 2
ER -
ID: 129670499