Beta-cell dysfunction induced by non-cytotoxic concentrations of Interleukin-1 beta is associated with changes in expression of beta-cell maturity genes and associated histone modifications

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Adriana Ibarra Urizar, Michala Prause, Matthew Wortham, Yinghui Sui, Peter Thams, Maike Sander, Gitte Lund Christensen, Nils Billestrup

Decreased insulin secretory capacity in Type 2 diabetes mellitus is associated with beta-cell dedifferentiation and inflammation. We hypothesize that prolonged exposure of beta-cells to low concentrations of IL-1 beta induce beta-cell dedifferentiation characterized by impaired glucose-stimulated insulin secretion, reduced expression of key beta-cell genes and changes in histone modifications at gene loci known to affect beta-cell function.

Ten days exposure to IL-1 beta at non-cytotoxic concentrations reduced insulin secretion and beta-cell proliferation and decreased expression of key beta-cell identity genes, including MafA and Ucn3 and decreased H3K27ac at the gene loci, suggesting that inflammatory cytokines directly affects the epigenome. Following removal of IL-1 beta, beta-cell function was normalized and mRNA expression of beta-cell identity genes, such as insulin and Ucn3 returned to pre-stimulation levels.

Our findings indicate that prolonged exposure to low concentrations of IL-1 beta induces epigenetic changes associated with loss of beta-cell identity as observed in Type 2 diabetes
Original languageEnglish
Article number110524
JournalMolecular and Cellular Endocrinology
Volume496
Number of pages12
ISSN0303-7207
DOIs
Publication statusPublished - 2019

    Research areas

  • Beta-cell dysfunction, Histone modifications, Beta-cell dedifferentiation, Cytokines, Proliferation, Insulin secretion

ID: 228452365