Substance P and neurokinin A are codistributed and colocalized in the porcine gastrointestinal tract

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Substance P and neurokinin A are codistributed and colocalized in the porcine gastrointestinal tract. / Poulsen, Steen Seier; Bersani, M; Holst, J J; Schmidt, Peter; N. Rasmussen, Torben.

In: Peptides, Vol. 12, No. 5, 1991, p. 963-73.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Poulsen, SS, Bersani, M, Holst, JJ, Schmidt, P & N. Rasmussen, T 1991, 'Substance P and neurokinin A are codistributed and colocalized in the porcine gastrointestinal tract', Peptides, vol. 12, no. 5, pp. 963-73. https://doi.org/10.1016/0196-9781(91)90045-Q

APA

Poulsen, S. S., Bersani, M., Holst, J. J., Schmidt, P., & N. Rasmussen, T. (1991). Substance P and neurokinin A are codistributed and colocalized in the porcine gastrointestinal tract. Peptides, 12(5), 963-73. https://doi.org/10.1016/0196-9781(91)90045-Q

Vancouver

Poulsen SS, Bersani M, Holst JJ, Schmidt P, N. Rasmussen T. Substance P and neurokinin A are codistributed and colocalized in the porcine gastrointestinal tract. Peptides. 1991;12(5):963-73. https://doi.org/10.1016/0196-9781(91)90045-Q

Author

Poulsen, Steen Seier ; Bersani, M ; Holst, J J ; Schmidt, Peter ; N. Rasmussen, Torben. / Substance P and neurokinin A are codistributed and colocalized in the porcine gastrointestinal tract. In: Peptides. 1991 ; Vol. 12, No. 5. pp. 963-73.

Bibtex

@article{ca9a2b60ad164e8a8bcefb3692b12307,
title = "Substance P and neurokinin A are codistributed and colocalized in the porcine gastrointestinal tract",
abstract = "Immunoreactive substance P and neurokinin A were measured with radioimmunoassay in extracts of different segments of porcine gastrointestinal tract using C-terminally directed antisera. In all segments, the concentrations of substance P and neurokinin A were similar. The largest concentrations of both peptides were found in the mid-colon. By gel chromatography and reversed-phase high pressure liquid chromatography the immunoreactivity in extracts from ileum eluted as homogenous peptides at the positions of synthetic substance P and neurokinin A, respectively. No neurokinin B was found. By immunohistochemistry of porcine duodenum, jejunum, ileum and mid-colon, identical localization patterns were found for substance P and neurokinin A, and the two peptides demonstrated by double immunofluorescence to be colocalized in the enteric nervous system of the ileum. We conclude that the tachykinins substance P and neurokinin A are codistributed and colocalized in the procine gastrointestinal tract and suggest that the two peptides are produced from a common precursor, beta- and/or gamma-preprotachykinin, in the same neurons.",
keywords = "Animals, Digestive System, Fluorescent Antibody Technique, Immune Sera, Muscle, Smooth, Myenteric Plexus, Nerve Fibers, Neurokinin A, Organ Specificity, Substance P, Swine",
author = "Poulsen, {Steen Seier} and M Bersani and Holst, {J J} and Peter Schmidt and {N. Rasmussen}, Torben",
year = "1991",
doi = "10.1016/0196-9781(91)90045-Q",
language = "English",
volume = "12",
pages = "963--73",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Substance P and neurokinin A are codistributed and colocalized in the porcine gastrointestinal tract

AU - Poulsen, Steen Seier

AU - Bersani, M

AU - Holst, J J

AU - Schmidt, Peter

AU - N. Rasmussen, Torben

PY - 1991

Y1 - 1991

N2 - Immunoreactive substance P and neurokinin A were measured with radioimmunoassay in extracts of different segments of porcine gastrointestinal tract using C-terminally directed antisera. In all segments, the concentrations of substance P and neurokinin A were similar. The largest concentrations of both peptides were found in the mid-colon. By gel chromatography and reversed-phase high pressure liquid chromatography the immunoreactivity in extracts from ileum eluted as homogenous peptides at the positions of synthetic substance P and neurokinin A, respectively. No neurokinin B was found. By immunohistochemistry of porcine duodenum, jejunum, ileum and mid-colon, identical localization patterns were found for substance P and neurokinin A, and the two peptides demonstrated by double immunofluorescence to be colocalized in the enteric nervous system of the ileum. We conclude that the tachykinins substance P and neurokinin A are codistributed and colocalized in the procine gastrointestinal tract and suggest that the two peptides are produced from a common precursor, beta- and/or gamma-preprotachykinin, in the same neurons.

AB - Immunoreactive substance P and neurokinin A were measured with radioimmunoassay in extracts of different segments of porcine gastrointestinal tract using C-terminally directed antisera. In all segments, the concentrations of substance P and neurokinin A were similar. The largest concentrations of both peptides were found in the mid-colon. By gel chromatography and reversed-phase high pressure liquid chromatography the immunoreactivity in extracts from ileum eluted as homogenous peptides at the positions of synthetic substance P and neurokinin A, respectively. No neurokinin B was found. By immunohistochemistry of porcine duodenum, jejunum, ileum and mid-colon, identical localization patterns were found for substance P and neurokinin A, and the two peptides demonstrated by double immunofluorescence to be colocalized in the enteric nervous system of the ileum. We conclude that the tachykinins substance P and neurokinin A are codistributed and colocalized in the procine gastrointestinal tract and suggest that the two peptides are produced from a common precursor, beta- and/or gamma-preprotachykinin, in the same neurons.

KW - Animals

KW - Digestive System

KW - Fluorescent Antibody Technique

KW - Immune Sera

KW - Muscle, Smooth

KW - Myenteric Plexus

KW - Nerve Fibers

KW - Neurokinin A

KW - Organ Specificity

KW - Substance P

KW - Swine

U2 - 10.1016/0196-9781(91)90045-Q

DO - 10.1016/0196-9781(91)90045-Q

M3 - Journal article

C2 - 1724799

VL - 12

SP - 963

EP - 973

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 5

ER -

ID: 47487840