Reversed-phase high-performance liquid chromatographic analyses of insulin biosynthesis in isolated rat and mouse islets
Research output: Contribution to journal › Journal article › Research › peer-review
Two RP-HPLC systems were developed for the separation of the products of the conversion of proinsulin into insulin in rat and mouse islets, including proinsulin I and II. Peaks were identified by microsequencing and radiosequencing. It was confirmed that mouse C-peptide I has a two amino acid deletion compared to rat C-peptide I. A marked species difference in the ratio between insulin I and II was observed, i.e., 2:1 in the rat and 1:2 in the mouse. Pulse-chase experiments in rat islets have demonstrated that the ratio between insulin I and II in newly synthesized insulin is higher than that of the stored insulin, indicating a slower conversion rate of proinsulin II compared to proinsulin I.
Original language | English |
---|---|
Journal | Journal of Chromatography A |
Volume | 462 |
Pages (from-to) | 243-54 |
Number of pages | 12 |
ISSN | 0021-9673 |
Publication status | Published - 13 Jan 1989 |
- Alleles, Amino Acid Sequence, Animals, C-Peptide, Chromatography, High Pressure Liquid, Insulin, Islets of Langerhans, Mice, Mice, Inbred Strains, Molecular Sequence Data, Proinsulin, Rats, Rats, Inbred Strains
Research areas
ID: 47974424