Metabolism of i.v. administered 45 kDa epidermal growth factor in the rat

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Standard

Metabolism of i.v. administered 45 kDa epidermal growth factor in the rat. / Jørgensen, P E; Nexø, Ebba; Raaberg, Lasse; Poulsen, Steen Seier.

In: Regulatory Peptides, Vol. 30, No. 2, 10.09.1990, p. 113-23.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Jørgensen, PE, Nexø, E, Raaberg, L & Poulsen, SS 1990, 'Metabolism of i.v. administered 45 kDa epidermal growth factor in the rat', Regulatory Peptides, vol. 30, no. 2, pp. 113-23.

APA

Jørgensen, P. E., Nexø, E., Raaberg, L., & Poulsen, S. S. (1990). Metabolism of i.v. administered 45 kDa epidermal growth factor in the rat. Regulatory Peptides, 30(2), 113-23.

Vancouver

Jørgensen PE, Nexø E, Raaberg L, Poulsen SS. Metabolism of i.v. administered 45 kDa epidermal growth factor in the rat. Regulatory Peptides. 1990 Sep 10;30(2):113-23.

Author

Jørgensen, P E ; Nexø, Ebba ; Raaberg, Lasse ; Poulsen, Steen Seier. / Metabolism of i.v. administered 45 kDa epidermal growth factor in the rat. In: Regulatory Peptides. 1990 ; Vol. 30, No. 2. pp. 113-23.

Bibtex

@article{84fca5542b204b99a6e39609183e205f,
title = "Metabolism of i.v. administered 45 kDa epidermal growth factor in the rat",
abstract = "The 45 kDa epidermal growth factor (EGF-(45 kDa)) has been purified from rat urine. We have investigated the distribution and the processing of i.v. injected 125I-labeled EGF-(45 kDa) in the rat. 2.5 min after the i.v. injection only 12% of the label remained in the blood. Most of the label was found in the liver (54%), in the kidneys (7%) and in the skin (4%). The submandibular glands, stomach, small intestine, colon, spleen and lungs contained 1% or less of the radioactivity. Some of the 125I-EGF-(45 kDa) was processed to 125I-EGF-(6 kDa) immunoreactivity in the liver and in the kidneys. The kidneys excreted 125I-EGF-(45 kDa) in the urine, but we were not able to demonstrate 125I-EGF-(6 kDa) in urine. In conclusion, this study shows that homologous EGF-(45 kDa) is cleared from the circulation of rats within a few minutes, mainly by the liver and the kidneys. In vivo both the liver and the kidneys are able to process some of the EGF-(45 kDa) to EGF-(6 kDa) immunoreactivity.",
keywords = "Animals, Epidermal Growth Factor, Injections, Intravenous, Kidney, Liver, Male, Molecular Weight, Protein Precursors, Rats, Rats, Inbred Strains, Skin, Tissue Distribution",
author = "J{\o}rgensen, {P E} and Ebba Nex{\o} and Lasse Raaberg and Poulsen, {Steen Seier}",
year = "1990",
month = sep,
day = "10",
language = "English",
volume = "30",
pages = "113--23",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Metabolism of i.v. administered 45 kDa epidermal growth factor in the rat

AU - Jørgensen, P E

AU - Nexø, Ebba

AU - Raaberg, Lasse

AU - Poulsen, Steen Seier

PY - 1990/9/10

Y1 - 1990/9/10

N2 - The 45 kDa epidermal growth factor (EGF-(45 kDa)) has been purified from rat urine. We have investigated the distribution and the processing of i.v. injected 125I-labeled EGF-(45 kDa) in the rat. 2.5 min after the i.v. injection only 12% of the label remained in the blood. Most of the label was found in the liver (54%), in the kidneys (7%) and in the skin (4%). The submandibular glands, stomach, small intestine, colon, spleen and lungs contained 1% or less of the radioactivity. Some of the 125I-EGF-(45 kDa) was processed to 125I-EGF-(6 kDa) immunoreactivity in the liver and in the kidneys. The kidneys excreted 125I-EGF-(45 kDa) in the urine, but we were not able to demonstrate 125I-EGF-(6 kDa) in urine. In conclusion, this study shows that homologous EGF-(45 kDa) is cleared from the circulation of rats within a few minutes, mainly by the liver and the kidneys. In vivo both the liver and the kidneys are able to process some of the EGF-(45 kDa) to EGF-(6 kDa) immunoreactivity.

AB - The 45 kDa epidermal growth factor (EGF-(45 kDa)) has been purified from rat urine. We have investigated the distribution and the processing of i.v. injected 125I-labeled EGF-(45 kDa) in the rat. 2.5 min after the i.v. injection only 12% of the label remained in the blood. Most of the label was found in the liver (54%), in the kidneys (7%) and in the skin (4%). The submandibular glands, stomach, small intestine, colon, spleen and lungs contained 1% or less of the radioactivity. Some of the 125I-EGF-(45 kDa) was processed to 125I-EGF-(6 kDa) immunoreactivity in the liver and in the kidneys. The kidneys excreted 125I-EGF-(45 kDa) in the urine, but we were not able to demonstrate 125I-EGF-(6 kDa) in urine. In conclusion, this study shows that homologous EGF-(45 kDa) is cleared from the circulation of rats within a few minutes, mainly by the liver and the kidneys. In vivo both the liver and the kidneys are able to process some of the EGF-(45 kDa) to EGF-(6 kDa) immunoreactivity.

KW - Animals

KW - Epidermal Growth Factor

KW - Injections, Intravenous

KW - Kidney

KW - Liver

KW - Male

KW - Molecular Weight

KW - Protein Precursors

KW - Rats

KW - Rats, Inbred Strains

KW - Skin

KW - Tissue Distribution

M3 - Journal article

C2 - 2274677

VL - 30

SP - 113

EP - 123

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

IS - 2

ER -

ID: 47488139