GRP-producing nerves control antral somatostatin and gastrin secretion in pigs
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GRP-producing nerves control antral somatostatin and gastrin secretion in pigs. / Holst, J J; Orskov, C; Poulsen, Steen Seier.
In: American Journal of Physiology (Consolidated), Vol. 253, No. 6 Pt 1, 12.1987, p. G767-74.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - GRP-producing nerves control antral somatostatin and gastrin secretion in pigs
AU - Holst, J J
AU - Orskov, C
AU - Poulsen, Steen Seier
PY - 1987/12
Y1 - 1987/12
N2 - By immunohistochemistry, nerve fibers containing gastrin-releasing polypeptide (GRP)-like immunoreactivity were identified close to the somatostatin (SS)-producing cells of the gastric antral mucosa. We, therefore, studied the possible role of GRP in the control of antral SS secretion by use of isolated perfused pig antrum with intact vagus nerve supply. Electrical stimulation of the vagus nerves at 4 Hz increased the antral release of GRP up to 10-fold and increased SS output 2- to 3-fold. Atropine at 10(-6) M had no effect on these responses. Intra-arterial GRP increased SS secretion significantly at 10(-10) M and eightfold at 10(-8) M, whereas gastrin secretion was stimulated significantly at 10(-11) M and maximally at 10(-10) M and inhibited at 10(-8) M. Preperfusion with a GRP antagonist ([D-Arg1,D-Pro2,D-Trp7,9,Leu11]substance P) or Fab fragments of antibodies against GRP abolished the effects of vagus stimulation on gastrin and somatostatin output. Gastrin in concentrations up to 10(-7) M was without effect on SS secretion. We conclude that electrical stimulation of the vagus nerves increases antral SS gastrin secretion and that GRP is a likely transmitter.
AB - By immunohistochemistry, nerve fibers containing gastrin-releasing polypeptide (GRP)-like immunoreactivity were identified close to the somatostatin (SS)-producing cells of the gastric antral mucosa. We, therefore, studied the possible role of GRP in the control of antral SS secretion by use of isolated perfused pig antrum with intact vagus nerve supply. Electrical stimulation of the vagus nerves at 4 Hz increased the antral release of GRP up to 10-fold and increased SS output 2- to 3-fold. Atropine at 10(-6) M had no effect on these responses. Intra-arterial GRP increased SS secretion significantly at 10(-10) M and eightfold at 10(-8) M, whereas gastrin secretion was stimulated significantly at 10(-11) M and maximally at 10(-10) M and inhibited at 10(-8) M. Preperfusion with a GRP antagonist ([D-Arg1,D-Pro2,D-Trp7,9,Leu11]substance P) or Fab fragments of antibodies against GRP abolished the effects of vagus stimulation on gastrin and somatostatin output. Gastrin in concentrations up to 10(-7) M was without effect on SS secretion. We conclude that electrical stimulation of the vagus nerves increases antral SS gastrin secretion and that GRP is a likely transmitter.
KW - Animals
KW - Atropine
KW - Electric Stimulation
KW - Gastrin-Releasing Peptide
KW - Gastrins
KW - Immunoenzyme Techniques
KW - Immunologic Techniques
KW - Neurotransmitter Agents
KW - Peptides
KW - Perfusion
KW - Secretory Rate
KW - Somatostatin
KW - Stomach
KW - Swine
KW - Vagus Nerve
M3 - Journal article
C2 - 2892416
VL - 253
SP - G767-74
JO - American Journal of Physiology - Cell Physiology
JF - American Journal of Physiology - Cell Physiology
SN - 0363-6143
IS - 6 Pt 1
ER -
ID: 47488455