Xanthine oxidase activity and free radical generation in patients with sepsis syndrome

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Xanthine oxidase activity and free radical generation in patients with sepsis syndrome. / Galley, H F; Davies, Michael Jonathan; Webster, N R.

In: Critical Care Medicine, Vol. 24, No. 10, 1996, p. 1649-53.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Galley, HF, Davies, MJ & Webster, NR 1996, 'Xanthine oxidase activity and free radical generation in patients with sepsis syndrome', Critical Care Medicine, vol. 24, no. 10, pp. 1649-53.

APA

Galley, H. F., Davies, M. J., & Webster, N. R. (1996). Xanthine oxidase activity and free radical generation in patients with sepsis syndrome. Critical Care Medicine, 24(10), 1649-53.

Vancouver

Galley HF, Davies MJ, Webster NR. Xanthine oxidase activity and free radical generation in patients with sepsis syndrome. Critical Care Medicine. 1996;24(10):1649-53.

Author

Galley, H F ; Davies, Michael Jonathan ; Webster, N R. / Xanthine oxidase activity and free radical generation in patients with sepsis syndrome. In: Critical Care Medicine. 1996 ; Vol. 24, No. 10. pp. 1649-53.

Bibtex

@article{0d4001ea09ae4053adae8fb681db9f35,
title = "Xanthine oxidase activity and free radical generation in patients with sepsis syndrome",
abstract = "OBJECTIVE: To determine xanthine oxidase activity, free radical concentrations, and lipid peroxidation in patients with sepsis syndrome compared with noninfected critically ill patients.DESIGN: A prospective observational study.SETTING: A nine-bed intensive care unit in a university teaching hospital trust.PATIENTS: Fourteen consecutive patients who met the established criteria for sepsis syndrome with multiple organ dysfunction syndrome, and ten noninfected critically ill patients were studied.INTERVENTIONS: None.MEASUREMENTS AND MAIN RESULTS: Xanthine oxidase activity was increased in septic patients compared with both healthy volunteers (p < .01) and noninfected patients (p < .05), and was highest in the six patients who survived (p < .05). Lipid peroxides were increased in both septic patients (p < .001) and nonseptic controls (p < .001). Xanthine oxidase activity did not relate to the Acute Physiology and Chronic Health Evaluation (APACHE) II score or to the presence of organ dysfunction. The mean ascorbyl radical concentration (arbitrary units) determined by electron paramagnetic resonance following spin trapping was increased in patients compared with healthy subjects (p < .05).CONCLUSIONS: Patients with sepsis have xanthine oxidase activation, high free-radical concentrations, and evidence of free radical damage. The finding that xanthine oxidase activity was lower in those patients who died, coupled with increased lactate concentrations implies more severe ischemia with incomplete reperfusion resulting in less xanthine oxidase {"}wash out{"} into the circulation. Increased ascorbyl radical concentrations may be due to an increased radical generation and oxidant scavenging, but appears to be unrelated to xanthine oxidase activity within the circulation.",
keywords = "APACHE, Adult, Aged, Aged, 80 and over, Critical Illness, Female, Free Radicals, Humans, Lactates, Lipid Peroxidation, Male, Middle Aged, Multiple Organ Failure, Prospective Studies, Survival Rate, Systemic Inflammatory Response Syndrome, Xanthine Oxidase",
author = "Galley, {H F} and Davies, {Michael Jonathan} and Webster, {N R}",
year = "1996",
language = "English",
volume = "24",
pages = "1649--53",
journal = "Critical Care Medicine",
issn = "0090-3493",
publisher = "Lippincott Williams & Wilkins",
number = "10",

}

RIS

TY - JOUR

T1 - Xanthine oxidase activity and free radical generation in patients with sepsis syndrome

AU - Galley, H F

AU - Davies, Michael Jonathan

AU - Webster, N R

PY - 1996

Y1 - 1996

N2 - OBJECTIVE: To determine xanthine oxidase activity, free radical concentrations, and lipid peroxidation in patients with sepsis syndrome compared with noninfected critically ill patients.DESIGN: A prospective observational study.SETTING: A nine-bed intensive care unit in a university teaching hospital trust.PATIENTS: Fourteen consecutive patients who met the established criteria for sepsis syndrome with multiple organ dysfunction syndrome, and ten noninfected critically ill patients were studied.INTERVENTIONS: None.MEASUREMENTS AND MAIN RESULTS: Xanthine oxidase activity was increased in septic patients compared with both healthy volunteers (p < .01) and noninfected patients (p < .05), and was highest in the six patients who survived (p < .05). Lipid peroxides were increased in both septic patients (p < .001) and nonseptic controls (p < .001). Xanthine oxidase activity did not relate to the Acute Physiology and Chronic Health Evaluation (APACHE) II score or to the presence of organ dysfunction. The mean ascorbyl radical concentration (arbitrary units) determined by electron paramagnetic resonance following spin trapping was increased in patients compared with healthy subjects (p < .05).CONCLUSIONS: Patients with sepsis have xanthine oxidase activation, high free-radical concentrations, and evidence of free radical damage. The finding that xanthine oxidase activity was lower in those patients who died, coupled with increased lactate concentrations implies more severe ischemia with incomplete reperfusion resulting in less xanthine oxidase "wash out" into the circulation. Increased ascorbyl radical concentrations may be due to an increased radical generation and oxidant scavenging, but appears to be unrelated to xanthine oxidase activity within the circulation.

AB - OBJECTIVE: To determine xanthine oxidase activity, free radical concentrations, and lipid peroxidation in patients with sepsis syndrome compared with noninfected critically ill patients.DESIGN: A prospective observational study.SETTING: A nine-bed intensive care unit in a university teaching hospital trust.PATIENTS: Fourteen consecutive patients who met the established criteria for sepsis syndrome with multiple organ dysfunction syndrome, and ten noninfected critically ill patients were studied.INTERVENTIONS: None.MEASUREMENTS AND MAIN RESULTS: Xanthine oxidase activity was increased in septic patients compared with both healthy volunteers (p < .01) and noninfected patients (p < .05), and was highest in the six patients who survived (p < .05). Lipid peroxides were increased in both septic patients (p < .001) and nonseptic controls (p < .001). Xanthine oxidase activity did not relate to the Acute Physiology and Chronic Health Evaluation (APACHE) II score or to the presence of organ dysfunction. The mean ascorbyl radical concentration (arbitrary units) determined by electron paramagnetic resonance following spin trapping was increased in patients compared with healthy subjects (p < .05).CONCLUSIONS: Patients with sepsis have xanthine oxidase activation, high free-radical concentrations, and evidence of free radical damage. The finding that xanthine oxidase activity was lower in those patients who died, coupled with increased lactate concentrations implies more severe ischemia with incomplete reperfusion resulting in less xanthine oxidase "wash out" into the circulation. Increased ascorbyl radical concentrations may be due to an increased radical generation and oxidant scavenging, but appears to be unrelated to xanthine oxidase activity within the circulation.

KW - APACHE

KW - Adult

KW - Aged

KW - Aged, 80 and over

KW - Critical Illness

KW - Female

KW - Free Radicals

KW - Humans

KW - Lactates

KW - Lipid Peroxidation

KW - Male

KW - Middle Aged

KW - Multiple Organ Failure

KW - Prospective Studies

KW - Survival Rate

KW - Systemic Inflammatory Response Syndrome

KW - Xanthine Oxidase

M3 - Journal article

C2 - 8874300

VL - 24

SP - 1649

EP - 1653

JO - Critical Care Medicine

JF - Critical Care Medicine

SN - 0090-3493

IS - 10

ER -

ID: 138287122