Whole-genome amplified DNA from stored dried blood spots is reliable in high resolution melting curve and sequencing analysis
Research output: Contribution to journal › Journal article › Research › peer-review
BACKGROUND: The use of dried blood spots (DBS) samples in genomic workup has been limited by the relative low amounts of genomic DNA (gDNA) they contain. It remains to be proven that whole genome amplified DNA (wgaDNA) from stored DBS samples, constitutes a reliable alternative to gDNA.We wanted to compare melting curves and sequencing results from wgaDNA derived from DBS samples with gDNA derived from whole blood.
METHODS: gDNA was extracted from whole blood obtained from 10 patients with lone atrial fibrillation (mean age 22.3 years). From their newborn DBS samples, stored at -24°C, genomic DNA was extracted and whole-genome amplified in triplicates. Using high resolution melting curve analysis and direct sequencing in both wgaDNA and gDNA samples, all coding regions and adjacent intron regions of the genes SCN5A and KCNA5 were investigated.
RESULTS: Altered melting curves was present in 85 of wgaDNA samples and 81 of gDNA samples. Sequence analysis identified a total of 31 variants in the 10 wgaDNA samples. The same 31 variants were found in the exact same pattern of samples in the gDNA group. There was no false positive or negative sequence variation in the wgaDNA group.
CONCLUSIONS: The use of DNA amplified in triplicates from DBS samples is reliable and can be used both for high resolution curve melting analysis as well as direct sequence analysis. DBS samples therefore can serve as an alternative to whole blood in sequence analysis.
|BMC Medical Genetics
|Published - 9 Feb 2011
- Adult, Blood Preservation/methods, Blood Specimen Collection/methods, Genome, Genome, Human/genetics, Genomics/methods, Hot Temperature, Humans, Infant, Newborn, Kv1.5 Potassium Channel/genetics, Muscle Proteins/genetics, NAV1.5 Voltage-Gated Sodium Channel, Nucleic Acid Amplification Techniques, Phase Transition, Sodium Channels/genetics, Specimen Handling/methods