Trafficking of the IKs -Complex in MDCK Cells: Site of Subunit Assembly and Determinants of Polarized Localization

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Trafficking of the IKs -Complex in MDCK Cells : Site of Subunit Assembly and Determinants of Polarized Localization. / David, Jens-Peter; Andersen, Martin N; Olesen, Søren-Peter; Rasmussen, Hanne B; Schmitt, Nicole.

In: Traffic, Vol. 14, No. 4, 04.2013, p. 399-411.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

David, J-P, Andersen, MN, Olesen, S-P, Rasmussen, HB & Schmitt, N 2013, 'Trafficking of the IKs -Complex in MDCK Cells: Site of Subunit Assembly and Determinants of Polarized Localization', Traffic, vol. 14, no. 4, pp. 399-411. https://doi.org/10.1111/tra.12042

APA

David, J-P., Andersen, M. N., Olesen, S-P., Rasmussen, H. B., & Schmitt, N. (2013). Trafficking of the IKs -Complex in MDCK Cells: Site of Subunit Assembly and Determinants of Polarized Localization. Traffic, 14(4), 399-411. https://doi.org/10.1111/tra.12042

Vancouver

David J-P, Andersen MN, Olesen S-P, Rasmussen HB, Schmitt N. Trafficking of the IKs -Complex in MDCK Cells: Site of Subunit Assembly and Determinants of Polarized Localization. Traffic. 2013 Apr;14(4):399-411. https://doi.org/10.1111/tra.12042

Author

David, Jens-Peter ; Andersen, Martin N ; Olesen, Søren-Peter ; Rasmussen, Hanne B ; Schmitt, Nicole. / Trafficking of the IKs -Complex in MDCK Cells : Site of Subunit Assembly and Determinants of Polarized Localization. In: Traffic. 2013 ; Vol. 14, No. 4. pp. 399-411.

Bibtex

@article{db252f701f354dce9e12b03d81bbd128,
title = "Trafficking of the IKs -Complex in MDCK Cells: Site of Subunit Assembly and Determinants of Polarized Localization",
abstract = "The voltage-gated potassium channel K 7.1 is regulated by non-pore forming regulatory KCNE {\ss}-subunits. Together with KCNE1, it forms the slowly activating delayed rectifier potassium current I . However, where the subunits assemble and which of the subunits determines localization of the I -complex has not been unequivocally resolved yet. We employed trafficking-deficient K 7.1 and KCNE1 mutants to investigate I trafficking using the polarized Madin-Darby Canine Kidney cell line. We find that the assembly happens early in the secretory pathway but provide three lines of evidence that it takes place in a post-endoplasmic reticulum compartment. We demonstrate that K 7.1 targets the I -complex to the basolateral membrane, but that KCNE1 can redirect the complex to the apical membrane upon mutation of critical K 7.1 basolateral targeting signals. Our data provide a possible explanation to the fact that K 7.1 can be localized apically or basolaterally in different epithelial tissues and offer a solution to divergent literature results regarding the effect of KCNE subunits on the subcellular localization of K 7.1/KCNE complexes.",
author = "Jens-Peter David and Andersen, {Martin N} and S{\o}ren-Peter Olesen and Rasmussen, {Hanne B} and Nicole Schmitt",
note = "{\circledC} 2013 John Wiley & Sons A/S.",
year = "2013",
month = "4",
doi = "10.1111/tra.12042",
language = "English",
volume = "14",
pages = "399--411",
journal = "Traffic",
issn = "1398-9219",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - Trafficking of the IKs -Complex in MDCK Cells

T2 - Site of Subunit Assembly and Determinants of Polarized Localization

AU - David, Jens-Peter

AU - Andersen, Martin N

AU - Olesen, Søren-Peter

AU - Rasmussen, Hanne B

AU - Schmitt, Nicole

N1 - © 2013 John Wiley & Sons A/S.

PY - 2013/4

Y1 - 2013/4

N2 - The voltage-gated potassium channel K 7.1 is regulated by non-pore forming regulatory KCNE ß-subunits. Together with KCNE1, it forms the slowly activating delayed rectifier potassium current I . However, where the subunits assemble and which of the subunits determines localization of the I -complex has not been unequivocally resolved yet. We employed trafficking-deficient K 7.1 and KCNE1 mutants to investigate I trafficking using the polarized Madin-Darby Canine Kidney cell line. We find that the assembly happens early in the secretory pathway but provide three lines of evidence that it takes place in a post-endoplasmic reticulum compartment. We demonstrate that K 7.1 targets the I -complex to the basolateral membrane, but that KCNE1 can redirect the complex to the apical membrane upon mutation of critical K 7.1 basolateral targeting signals. Our data provide a possible explanation to the fact that K 7.1 can be localized apically or basolaterally in different epithelial tissues and offer a solution to divergent literature results regarding the effect of KCNE subunits on the subcellular localization of K 7.1/KCNE complexes.

AB - The voltage-gated potassium channel K 7.1 is regulated by non-pore forming regulatory KCNE ß-subunits. Together with KCNE1, it forms the slowly activating delayed rectifier potassium current I . However, where the subunits assemble and which of the subunits determines localization of the I -complex has not been unequivocally resolved yet. We employed trafficking-deficient K 7.1 and KCNE1 mutants to investigate I trafficking using the polarized Madin-Darby Canine Kidney cell line. We find that the assembly happens early in the secretory pathway but provide three lines of evidence that it takes place in a post-endoplasmic reticulum compartment. We demonstrate that K 7.1 targets the I -complex to the basolateral membrane, but that KCNE1 can redirect the complex to the apical membrane upon mutation of critical K 7.1 basolateral targeting signals. Our data provide a possible explanation to the fact that K 7.1 can be localized apically or basolaterally in different epithelial tissues and offer a solution to divergent literature results regarding the effect of KCNE subunits on the subcellular localization of K 7.1/KCNE complexes.

U2 - 10.1111/tra.12042

DO - 10.1111/tra.12042

M3 - Journal article

C2 - 23324056

VL - 14

SP - 399

EP - 411

JO - Traffic

JF - Traffic

SN - 1398-9219

IS - 4

ER -

ID: 45080493