Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice
Research output: Contribution to journal › Journal article › Research › peer-review
Standard
Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice. / Købler, Carsten; Poulsen, Sarah S; Saber, Anne T; Jacobsen, Nicklas R; Wallin, Håkan; Yauk, Carole L; Halappanavar, Sabina; Vogel, Ulla; Qvortrup, Klaus; Mølhave, Kristian.
In: PLOS ONE, Vol. 10, No. 1, e0116481, 24.01.2015, p. 1-17.Research output: Contribution to journal › Journal article › Research › peer-review
Harvard
APA
Vancouver
Author
Bibtex
}
RIS
TY - JOUR
T1 - Time-Dependent Subcellular Distribution and Effects of Carbon Nanotubes in Lungs of Mice
AU - Købler, Carsten
AU - Poulsen, Sarah S
AU - Saber, Anne T
AU - Jacobsen, Nicklas R
AU - Wallin, Håkan
AU - Yauk, Carole L
AU - Halappanavar, Sabina
AU - Vogel, Ulla
AU - Qvortrup, Klaus
AU - Mølhave, Kristian
PY - 2015/1/24
Y1 - 2015/1/24
N2 - BACKGROUND AND METHODS: Pulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.RESULTS: TEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.CONCLUSION: Two very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP).
AB - BACKGROUND AND METHODS: Pulmonary deposited carbon nanotubes (CNTs) are cleared very slowly from the lung, but there is limited information on how CNTs interact with the lung tissue over time. To address this, three different multiwalled CNTs were intratracheally instilled into female C57BL/6 mice: one short (850 nm) and tangled, and two longer (4 μm and 5.7 μm) and thicker. We assessed the cellular interaction with these CNTs using transmission electron microscopy (TEM) 1, 3 and 28 days after instillation.RESULTS: TEM analysis revealed that the three CNTs followed the same overall progression pattern over time. Initially, CNTs were taken up either by a diffusion mechanism or via endocytosis. Then CNTs were agglomerated in vesicles in macrophages. Lastly, at 28 days post-exposure, evidence suggesting CNT escape from vesicle enclosures were found. The longer and thicker CNTs more often perturbed and escaped vesicular enclosures in macrophages compared to the smaller CNTs. Bronchoalveolar lavage (BAL) showed that the CNT exposure induced both an eosinophil influx and also eosinophilic crystalline pneumonia.CONCLUSION: Two very different types of multiwalled CNTs had very similar pattern of cellular interactions in lung tissue, with the longer and thicker CNTs resulting in more severe effects in terms of eosinophil influx and incidence of eosinophilic crystalline pneumonia (ECP).
U2 - 10.1371/journal.pone.0116481
DO - 10.1371/journal.pone.0116481
M3 - Journal article
C2 - 25615613
VL - 10
SP - 1
EP - 17
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 1
M1 - e0116481
ER -
ID: 130444128