Surface morphology of the endolymphatic duct in the rat. A scanning electron microscopy study

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Surface morphology of the endolymphatic duct in the rat. A scanning electron microscopy study. / Qvortrup, K; Rostgaard, Jørgen; Bretlau, P.

In: Annals of Otology, Rhinology and Laryngology, Vol. 104, No. 2, 02.1995, p. 120-6.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Qvortrup, K, Rostgaard, J & Bretlau, P 1995, 'Surface morphology of the endolymphatic duct in the rat. A scanning electron microscopy study', Annals of Otology, Rhinology and Laryngology, vol. 104, no. 2, pp. 120-6. https://doi.org/10.1177/000348949510400207

APA

Qvortrup, K., Rostgaard, J., & Bretlau, P. (1995). Surface morphology of the endolymphatic duct in the rat. A scanning electron microscopy study. Annals of Otology, Rhinology and Laryngology, 104(2), 120-6. https://doi.org/10.1177/000348949510400207

Vancouver

Qvortrup K, Rostgaard J, Bretlau P. Surface morphology of the endolymphatic duct in the rat. A scanning electron microscopy study. Annals of Otology, Rhinology and Laryngology. 1995 Feb;104(2):120-6. https://doi.org/10.1177/000348949510400207

Author

Qvortrup, K ; Rostgaard, Jørgen ; Bretlau, P. / Surface morphology of the endolymphatic duct in the rat. A scanning electron microscopy study. In: Annals of Otology, Rhinology and Laryngology. 1995 ; Vol. 104, No. 2. pp. 120-6.

Bibtex

@article{c270fea424ac40a18dca4dc3e2280088,
title = "Surface morphology of the endolymphatic duct in the rat. A scanning electron microscopy study",
abstract = "Following intracardiac vascular perfusion fixation of 8 rats with glutaraldehyde in a buffered and oxygenated blood substitute, the vestibular aqueduct and endolymphatic duct were opened by microsurgery of the resulting 16 temporal bones. Optimum preservation of the epithelium for scanning electron microscopy was attained by coating of the specimens with osmium tetroxide and thiocarbohydrazide followed by a continuous dehydration procedure. This technique permitted, for the first time, an investigation of the surface morphology of the epithelial cells in the endolymphatic duct. Three types of cells were identified with the scanning electron microscope. A polygonal and oblong epithelial cell was observed in the largest number throughout the duct, and in the juxtasaccular half of the duct, two additional types of epithelial cells were observed. The scanning electron microscopic observations are compared and discussed with reference to previous transmission electron microscopic studies of the endolymphatic duct.",
keywords = "Animals, Endolymphatic Duct, Epithelium, Glutaral, Microscopy, Electron, Scanning, Myocardial Reperfusion, Postoperative Period, Rats, Rats, Wistar, Journal Article, Research Support, Non-U.S. Gov't",
author = "K Qvortrup and J{\o}rgen Rostgaard and P Bretlau",
year = "1995",
month = feb,
doi = "10.1177/000348949510400207",
language = "English",
volume = "104",
pages = "120--6",
journal = "The Annals of otology, rhinology & laryngology. Supplement",
issn = "0096-8056",
publisher = "SAGE Publications",
number = "2",

}

RIS

TY - JOUR

T1 - Surface morphology of the endolymphatic duct in the rat. A scanning electron microscopy study

AU - Qvortrup, K

AU - Rostgaard, Jørgen

AU - Bretlau, P

PY - 1995/2

Y1 - 1995/2

N2 - Following intracardiac vascular perfusion fixation of 8 rats with glutaraldehyde in a buffered and oxygenated blood substitute, the vestibular aqueduct and endolymphatic duct were opened by microsurgery of the resulting 16 temporal bones. Optimum preservation of the epithelium for scanning electron microscopy was attained by coating of the specimens with osmium tetroxide and thiocarbohydrazide followed by a continuous dehydration procedure. This technique permitted, for the first time, an investigation of the surface morphology of the epithelial cells in the endolymphatic duct. Three types of cells were identified with the scanning electron microscope. A polygonal and oblong epithelial cell was observed in the largest number throughout the duct, and in the juxtasaccular half of the duct, two additional types of epithelial cells were observed. The scanning electron microscopic observations are compared and discussed with reference to previous transmission electron microscopic studies of the endolymphatic duct.

AB - Following intracardiac vascular perfusion fixation of 8 rats with glutaraldehyde in a buffered and oxygenated blood substitute, the vestibular aqueduct and endolymphatic duct were opened by microsurgery of the resulting 16 temporal bones. Optimum preservation of the epithelium for scanning electron microscopy was attained by coating of the specimens with osmium tetroxide and thiocarbohydrazide followed by a continuous dehydration procedure. This technique permitted, for the first time, an investigation of the surface morphology of the epithelial cells in the endolymphatic duct. Three types of cells were identified with the scanning electron microscope. A polygonal and oblong epithelial cell was observed in the largest number throughout the duct, and in the juxtasaccular half of the duct, two additional types of epithelial cells were observed. The scanning electron microscopic observations are compared and discussed with reference to previous transmission electron microscopic studies of the endolymphatic duct.

KW - Animals

KW - Endolymphatic Duct

KW - Epithelium

KW - Glutaral

KW - Microscopy, Electron, Scanning

KW - Myocardial Reperfusion

KW - Postoperative Period

KW - Rats

KW - Rats, Wistar

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1177/000348949510400207

DO - 10.1177/000348949510400207

M3 - Journal article

C2 - 7857013

VL - 104

SP - 120

EP - 126

JO - The Annals of otology, rhinology & laryngology. Supplement

JF - The Annals of otology, rhinology & laryngology. Supplement

SN - 0096-8056

IS - 2

ER -

ID: 176538607