Reduced skeletal muscle mitochondrial respiration and improved glucose metabolism in nondiabetic obese women during a very low calorie dietary intervention leading to rapid weight loss
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Reduced skeletal muscle mitochondrial respiration and improved glucose metabolism in nondiabetic obese women during a very low calorie dietary intervention leading to rapid weight loss. / Rabøl, Rasmus; Svendsen, Pernille F; Skovbro, Mette; Boushel, Robert; Haugaard, Steen B; Schjerling, Peter; Schrauwen, Patrick; Hesselink, Matthijs K C; Nilas, Lisbeth; Madsbad, Sten; Dela, Flemming; Rabøl, Rasmus; Svendsen, Pernille F; Skovbro, Mette; Boushel, Robert Christopher; Haugaard, Steen B; Schjerling, Peter; Schrauwen, Patrick; Hesselink, Matthijs K C; Nilas, Lisbeth; Madsbad, Sten; Dela, Flemming.
In: Metabolism - Clinical and Experimental, Vol. 58, No. 8, 2009, p. 1145-52.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Reduced skeletal muscle mitochondrial respiration and improved glucose metabolism in nondiabetic obese women during a very low calorie dietary intervention leading to rapid weight loss
AU - Rabøl, Rasmus
AU - Svendsen, Pernille F
AU - Skovbro, Mette
AU - Boushel, Robert
AU - Haugaard, Steen B
AU - Schjerling, Peter
AU - Schrauwen, Patrick
AU - Hesselink, Matthijs K C
AU - Nilas, Lisbeth
AU - Madsbad, Sten
AU - Dela, Flemming
AU - Rabøl, Rasmus
AU - Svendsen, Pernille F
AU - Skovbro, Mette
AU - Boushel, Robert Christopher
AU - Haugaard, Steen B
AU - Schjerling, Peter
AU - Schrauwen, Patrick
AU - Hesselink, Matthijs K C
AU - Nilas, Lisbeth
AU - Madsbad, Sten
AU - Dela, Flemming
PY - 2009
Y1 - 2009
N2 - Reduced oxidative capacity of skeletal muscle has been proposed to lead to accumulation of intramyocellular triglyceride (IMTG) and insulin resistance. We have measured mitochondrial respiration before and after a 10% low-calorie-induced weight loss in young obese women to examine the relationship between mitochondrial function, IMTG, and insulin resistance. Nine obese women (age, 32.3 years [SD, 3.0]; body mass index, 33.4 kg/m(2) [SD, 2.6]) completed a 53-day (SE, 3.8) very low calorie diet (VLCD) of 500 to 600 kcal/d without altering physical activity. The target of the intervention was a 10% weight loss; and measurements of mitochondrial respiration, IMTG, respiratory exchange ratio, citrate synthase activity, mitochondrial DNA copy number, plasma insulin, 2-hour oral glucose tolerance test, and free fatty acids were performed before and after weight loss. Mitochondrial respiration was measured in permeabilized muscle fibers using high-resolution respirometry. Average weight loss was 11.5% (P < .05), but the levels of IMTG remained unchanged. Fasting plasma glucose, plasma insulin homeostasis model assessment of insulin resistance, and insulin sensitivity index (composite) obtained during 2-hour oral glucose tolerance test improved significantly. Mitochondrial respiration per milligram tissue decreased by approximately 25% (P < .05), but citrate synthase activity and mitochondrial DNA copy number remained unchanged. Respiratory exchange ratio decreased from 0.87 (SE, 0.01) to 0.79 (SE, 0.02) (P < .05) as a sign of increased whole-body fat oxidation. Markers of insulin sensitivity improved after the very low calorie diet; but mitochondrial function decreased, and IMTG remained unchanged. Our results do not support a direct relationship between mitochondrial function and insulin resistance in young obese women and do not support a direct relationship between IMTG and insulin sensitivity in young obese women during weight loss.
AB - Reduced oxidative capacity of skeletal muscle has been proposed to lead to accumulation of intramyocellular triglyceride (IMTG) and insulin resistance. We have measured mitochondrial respiration before and after a 10% low-calorie-induced weight loss in young obese women to examine the relationship between mitochondrial function, IMTG, and insulin resistance. Nine obese women (age, 32.3 years [SD, 3.0]; body mass index, 33.4 kg/m(2) [SD, 2.6]) completed a 53-day (SE, 3.8) very low calorie diet (VLCD) of 500 to 600 kcal/d without altering physical activity. The target of the intervention was a 10% weight loss; and measurements of mitochondrial respiration, IMTG, respiratory exchange ratio, citrate synthase activity, mitochondrial DNA copy number, plasma insulin, 2-hour oral glucose tolerance test, and free fatty acids were performed before and after weight loss. Mitochondrial respiration was measured in permeabilized muscle fibers using high-resolution respirometry. Average weight loss was 11.5% (P < .05), but the levels of IMTG remained unchanged. Fasting plasma glucose, plasma insulin homeostasis model assessment of insulin resistance, and insulin sensitivity index (composite) obtained during 2-hour oral glucose tolerance test improved significantly. Mitochondrial respiration per milligram tissue decreased by approximately 25% (P < .05), but citrate synthase activity and mitochondrial DNA copy number remained unchanged. Respiratory exchange ratio decreased from 0.87 (SE, 0.01) to 0.79 (SE, 0.02) (P < .05) as a sign of increased whole-body fat oxidation. Markers of insulin sensitivity improved after the very low calorie diet; but mitochondrial function decreased, and IMTG remained unchanged. Our results do not support a direct relationship between mitochondrial function and insulin resistance in young obese women and do not support a direct relationship between IMTG and insulin sensitivity in young obese women during weight loss.
U2 - 10.1016/j.metabol.2009.03.014
DO - 10.1016/j.metabol.2009.03.014
M3 - Journal article
C2 - 19454354
VL - 58
SP - 1145
EP - 1152
JO - Metabolism
JF - Metabolism
SN - 0026-0495
IS - 8
ER -
ID: 12771702