Reactive species formed on proteins exposed to singlet oxygen

Research output: Contribution to journalJournal articleResearchpeer-review

Standard

Reactive species formed on proteins exposed to singlet oxygen. / Davies, Michael Jonathan.

In: Photochemical & Photobiological Sciences, Vol. 3, No. 1, 01.2004, p. 17-25.

Research output: Contribution to journalJournal articleResearchpeer-review

Harvard

Davies, MJ 2004, 'Reactive species formed on proteins exposed to singlet oxygen', Photochemical & Photobiological Sciences, vol. 3, no. 1, pp. 17-25. https://doi.org/10.1039/b307576c

APA

Davies, M. J. (2004). Reactive species formed on proteins exposed to singlet oxygen. Photochemical & Photobiological Sciences, 3(1), 17-25. https://doi.org/10.1039/b307576c

Vancouver

Davies MJ. Reactive species formed on proteins exposed to singlet oxygen. Photochemical & Photobiological Sciences. 2004 Jan;3(1):17-25. https://doi.org/10.1039/b307576c

Author

Davies, Michael Jonathan. / Reactive species formed on proteins exposed to singlet oxygen. In: Photochemical & Photobiological Sciences. 2004 ; Vol. 3, No. 1. pp. 17-25.

Bibtex

@article{0fdd8f6c0fb84775ad7e647b20fac168,
title = "Reactive species formed on proteins exposed to singlet oxygen",
abstract = "Singlet oxygen ((1)O(2)) is believed to be generated in biological systems by a range of endogenous processes (e.g. enzymatic and chemical reactions) and exogenous stimuli (e.g. UV or visible light in the presence of a sensitiser). Kinetic data is consistent with proteins being a major target for (1)O(2), with damage occurring preferentially at Trp, His, Tyr, Met, and Cys side-chains. Reaction with each of these residues gives rise to further reactive species. In the case of Trp and Tyr, initial poorly characterised endoperoxides are believed to undergo ring-opening reactions to give hydroperoxides, which can be reduced to the corresponding alcohols; other products arising from radical intermediates can also be generated, particularly in the presence of UV light and metal ions. With His side-chains, poorly characterised peroxides are also formed. Reaction with Met and Cys has been proposed to occur via zwitterionic peroxy intermediates. Peroxides are also generated on isolated proteins, and protein within intact cells, via(1)O(2)-mediated reactions. The peroxides formed on Trp, Tyr, and His peptides, as well as on proteins, have been shown to induce damage to other targets, with molecular oxidation of thiol residues an important reaction. This can result in the inactivation of cellular enzymes and the oxidation of other biological targets. Protein cross-linking and aggregation can also be induced by reactive species formed on photo-oxidised proteins, though the nature of the species that participate in such reactions is poorly understood. These secondary reactions, and particularly those involving hydroperoxides, may play a key role in the induction of secondary damage (bystander effects) in systems subject to photo-oxidation.",
keywords = "Amino Acids, Antioxidants, Peroxides, Proteins, Reactive Oxygen Species, Singlet Oxygen, Ultraviolet Rays",
author = "Davies, {Michael Jonathan}",
year = "2004",
month = "1",
doi = "10.1039/b307576c",
language = "English",
volume = "3",
pages = "17--25",
journal = "Photochemical & Photobiological Sciences",
issn = "1474-905X",
publisher = "Royal Society of Chemistry",
number = "1",

}

RIS

TY - JOUR

T1 - Reactive species formed on proteins exposed to singlet oxygen

AU - Davies, Michael Jonathan

PY - 2004/1

Y1 - 2004/1

N2 - Singlet oxygen ((1)O(2)) is believed to be generated in biological systems by a range of endogenous processes (e.g. enzymatic and chemical reactions) and exogenous stimuli (e.g. UV or visible light in the presence of a sensitiser). Kinetic data is consistent with proteins being a major target for (1)O(2), with damage occurring preferentially at Trp, His, Tyr, Met, and Cys side-chains. Reaction with each of these residues gives rise to further reactive species. In the case of Trp and Tyr, initial poorly characterised endoperoxides are believed to undergo ring-opening reactions to give hydroperoxides, which can be reduced to the corresponding alcohols; other products arising from radical intermediates can also be generated, particularly in the presence of UV light and metal ions. With His side-chains, poorly characterised peroxides are also formed. Reaction with Met and Cys has been proposed to occur via zwitterionic peroxy intermediates. Peroxides are also generated on isolated proteins, and protein within intact cells, via(1)O(2)-mediated reactions. The peroxides formed on Trp, Tyr, and His peptides, as well as on proteins, have been shown to induce damage to other targets, with molecular oxidation of thiol residues an important reaction. This can result in the inactivation of cellular enzymes and the oxidation of other biological targets. Protein cross-linking and aggregation can also be induced by reactive species formed on photo-oxidised proteins, though the nature of the species that participate in such reactions is poorly understood. These secondary reactions, and particularly those involving hydroperoxides, may play a key role in the induction of secondary damage (bystander effects) in systems subject to photo-oxidation.

AB - Singlet oxygen ((1)O(2)) is believed to be generated in biological systems by a range of endogenous processes (e.g. enzymatic and chemical reactions) and exogenous stimuli (e.g. UV or visible light in the presence of a sensitiser). Kinetic data is consistent with proteins being a major target for (1)O(2), with damage occurring preferentially at Trp, His, Tyr, Met, and Cys side-chains. Reaction with each of these residues gives rise to further reactive species. In the case of Trp and Tyr, initial poorly characterised endoperoxides are believed to undergo ring-opening reactions to give hydroperoxides, which can be reduced to the corresponding alcohols; other products arising from radical intermediates can also be generated, particularly in the presence of UV light and metal ions. With His side-chains, poorly characterised peroxides are also formed. Reaction with Met and Cys has been proposed to occur via zwitterionic peroxy intermediates. Peroxides are also generated on isolated proteins, and protein within intact cells, via(1)O(2)-mediated reactions. The peroxides formed on Trp, Tyr, and His peptides, as well as on proteins, have been shown to induce damage to other targets, with molecular oxidation of thiol residues an important reaction. This can result in the inactivation of cellular enzymes and the oxidation of other biological targets. Protein cross-linking and aggregation can also be induced by reactive species formed on photo-oxidised proteins, though the nature of the species that participate in such reactions is poorly understood. These secondary reactions, and particularly those involving hydroperoxides, may play a key role in the induction of secondary damage (bystander effects) in systems subject to photo-oxidation.

KW - Amino Acids

KW - Antioxidants

KW - Peroxides

KW - Proteins

KW - Reactive Oxygen Species

KW - Singlet Oxygen

KW - Ultraviolet Rays

U2 - 10.1039/b307576c

DO - 10.1039/b307576c

M3 - Journal article

C2 - 14743273

VL - 3

SP - 17

EP - 25

JO - Photochemical & Photobiological Sciences

JF - Photochemical & Photobiological Sciences

SN - 1474-905X

IS - 1

ER -

ID: 138274615