PKC and AMPK regulation of Kv1.5 potassium channels
Research output: Contribution to journal › Letter › peer-review
The voltage-gated Kv1.5 potassium channel, conducting the ultra-rapid rectifier K(+) current (IKur), is regulated through several pathways. Here we investigate if Kv1.5 surface expression is controlled by the 2 kinases PKC and AMPK, using Xenopus oocytes, MDCK cells and atrial derived HL-1 cells. By confocal microscopy combined with electrophysiology we demonstrate that PKC activation reduces Kv1.5 current, through a decrease in membrane expressed channels. AMPK activation was found to decrease the membrane expression in MDCK cells, but not in HL-1 cells and was furthermore shown to be dependent on co-expression of Nedd4-2 in Xenopus oocytes. These results indicate that Kv1.5 channels are regulated by both kinases, although through different molecular mechanisms in different cell systems.
Original language | English |
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Journal | Channels (Austin) |
Volume | 9 |
Issue number | 3 |
Pages (from-to) | 121-8 |
Number of pages | 8 |
ISSN | 1933-6950 |
DOIs | |
Publication status | Published - 2015 |
ID: 143670198