PKC and AMPK regulation of Kv1.5 potassium channels

Research output: Contribution to journalLetterResearchpeer-review

Martin Nybo Andersen, Lasse Skibsbye, Chuyi Tang, Frederic Petersen, Nanna MacAulay, Hanne Borger Rasmussen, Thomas Jespersen

The voltage-gated Kv1.5 potassium channel, conducting the ultra-rapid rectifier K(+) current (IKur), is regulated through several pathways. Here we investigate if Kv1.5 surface expression is controlled by the 2 kinases PKC and AMPK, using Xenopus oocytes, MDCK cells and atrial derived HL-1 cells. By confocal microscopy combined with electrophysiology we demonstrate that PKC activation reduces Kv1.5 current, through a decrease in membrane expressed channels. AMPK activation was found to decrease the membrane expression in MDCK cells, but not in HL-1 cells and was furthermore shown to be dependent on co-expression of Nedd4-2 in Xenopus oocytes. These results indicate that Kv1.5 channels are regulated by both kinases, although through different molecular mechanisms in different cell systems.

Original languageEnglish
JournalChannels (Austin)
Volume9
Issue number3
Pages (from-to)121-8
Number of pages8
ISSN1933-6950
DOIs
Publication statusPublished - 2015

ID: 143670198