Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119

Research output: Contribution to journal › Journal article › Research › peer-review

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Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119. / Arifin, Syamsul A.; Paternoster, Silvano; Carlessi, Rodrigo; Casari, Ilaria; Ekberg, Jeppe Hvidtfeldt; Maffucci, Tania; Newsholme, Philip; Rosenkilde, Mette M.; Falasca, Marco.

In: B B A - Molecular and Cell Biology of Lipids, Vol. 1863, No. 9, 2018, p. 1132-1141.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Arifin, SA, Paternoster, S, Carlessi, R, Casari, I, Ekberg, JH, Maffucci, T, Newsholme, P, Rosenkilde, MM & Falasca, M 2018, 'Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119', B B A - Molecular and Cell Biology of Lipids, vol. 1863, no. 9, pp. 1132-1141. https://doi.org/10.1016/j.bbalip.2018.06.007

APA

Arifin, S. A., Paternoster, S., Carlessi, R., Casari, I., Ekberg, J. H., Maffucci, T., Newsholme, P., Rosenkilde, M. M., & Falasca, M. (2018). Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119. B B A - Molecular and Cell Biology of Lipids, 1863(9), 1132-1141. https://doi.org/10.1016/j.bbalip.2018.06.007

Vancouver

Arifin SA, Paternoster S, Carlessi R, Casari I, Ekberg JH, Maffucci T et al. Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119. B B A - Molecular and Cell Biology of Lipids. 2018;1863(9):1132-1141. https://doi.org/10.1016/j.bbalip.2018.06.007

Author

Arifin, Syamsul A. ; Paternoster, Silvano ; Carlessi, Rodrigo ; Casari, Ilaria ; Ekberg, Jeppe Hvidtfeldt ; Maffucci, Tania ; Newsholme, Philip ; Rosenkilde, Mette M. ; Falasca, Marco. / Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119. In: B B A - Molecular and Cell Biology of Lipids. 2018 ; Vol. 1863, No. 9. pp. 1132-1141.

Bibtex

@article{7648685fca0c482f8d0ada73cf7896a2,
title = "Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119",
abstract = "The gastrointestinal tract is increasingly viewed as critical in controlling glucose metabolism, because of its role in secreting multiple glucoregulatory hormones, such as glucagon like peptide-1 (GLP-1). Here we investigate the molecular pathways behind the GLP-1- and insulin-secreting capabilities of a novel GPR119 agonist, Oleoyl-lysophosphatidylinositol (Oleoyl-LPI). Oleoyl-LPI is the only LPI species able to potently stimulate the release of GLP-1 in vitro, from murine and human L-cells, and ex-vivo from murine colonic primary cell preparations. Here we show that Oleoyl-LPI mediates GLP-1 secretion through GPR119 as this activity is ablated in cells lacking GPR119 and in colonic primary cell preparation from GPR119(-/-) mice. Similarly, Oleoyl-LPI-mediated insulin secretion is impaired in islets isolated from GPR119(-/-) mice. On the other hand, GLP-1 secretion is not impaired in cells lacking GPR55 in vitro or in colonic primary cell preparation from GPR55(-/-) mice. We therefore conclude that GPR119 is the Oleoyl-LPI receptor, upstream of ERK1/2 and cAMP/PKA/CREB pathways, where primarily ERK1/2 is required for GLP-1 secretion, while CREB activation appears dispensable.",
keywords = "Lysophosphatidylinositol (LPI), Glucagon-like peptide-1 (GLP-1), GPR119, GPR55, L-cells, Mixed colonic preparation",
author = "Arifin, {Syamsul A.} and Silvano Paternoster and Rodrigo Carlessi and Ilaria Casari and Ekberg, {Jeppe Hvidtfeldt} and Tania Maffucci and Philip Newsholme and Rosenkilde, {Mette M.} and Marco Falasca",
year = "2018",
doi = "10.1016/j.bbalip.2018.06.007",
language = "English",
volume = "1863",
pages = "1132--1141",
journal = "B B A - Molecular and Cell Biology of Lipids",
issn = "1388-1981",
publisher = "Elsevier",
number = "9",

}

RIS

TY - JOUR

T1 - Oleoyl-lysophosphatidylinositol enhances glucagon-like peptide-1 secretion from enteroendocrine L-cells through GPR119

AU - Arifin, Syamsul A.

AU - Paternoster, Silvano

AU - Carlessi, Rodrigo

AU - Casari, Ilaria

AU - Ekberg, Jeppe Hvidtfeldt

AU - Maffucci, Tania

AU - Newsholme, Philip

AU - Rosenkilde, Mette M.

AU - Falasca, Marco

PY - 2018

Y1 - 2018

N2 - The gastrointestinal tract is increasingly viewed as critical in controlling glucose metabolism, because of its role in secreting multiple glucoregulatory hormones, such as glucagon like peptide-1 (GLP-1). Here we investigate the molecular pathways behind the GLP-1- and insulin-secreting capabilities of a novel GPR119 agonist, Oleoyl-lysophosphatidylinositol (Oleoyl-LPI). Oleoyl-LPI is the only LPI species able to potently stimulate the release of GLP-1 in vitro, from murine and human L-cells, and ex-vivo from murine colonic primary cell preparations. Here we show that Oleoyl-LPI mediates GLP-1 secretion through GPR119 as this activity is ablated in cells lacking GPR119 and in colonic primary cell preparation from GPR119(-/-) mice. Similarly, Oleoyl-LPI-mediated insulin secretion is impaired in islets isolated from GPR119(-/-) mice. On the other hand, GLP-1 secretion is not impaired in cells lacking GPR55 in vitro or in colonic primary cell preparation from GPR55(-/-) mice. We therefore conclude that GPR119 is the Oleoyl-LPI receptor, upstream of ERK1/2 and cAMP/PKA/CREB pathways, where primarily ERK1/2 is required for GLP-1 secretion, while CREB activation appears dispensable.

AB - The gastrointestinal tract is increasingly viewed as critical in controlling glucose metabolism, because of its role in secreting multiple glucoregulatory hormones, such as glucagon like peptide-1 (GLP-1). Here we investigate the molecular pathways behind the GLP-1- and insulin-secreting capabilities of a novel GPR119 agonist, Oleoyl-lysophosphatidylinositol (Oleoyl-LPI). Oleoyl-LPI is the only LPI species able to potently stimulate the release of GLP-1 in vitro, from murine and human L-cells, and ex-vivo from murine colonic primary cell preparations. Here we show that Oleoyl-LPI mediates GLP-1 secretion through GPR119 as this activity is ablated in cells lacking GPR119 and in colonic primary cell preparation from GPR119(-/-) mice. Similarly, Oleoyl-LPI-mediated insulin secretion is impaired in islets isolated from GPR119(-/-) mice. On the other hand, GLP-1 secretion is not impaired in cells lacking GPR55 in vitro or in colonic primary cell preparation from GPR55(-/-) mice. We therefore conclude that GPR119 is the Oleoyl-LPI receptor, upstream of ERK1/2 and cAMP/PKA/CREB pathways, where primarily ERK1/2 is required for GLP-1 secretion, while CREB activation appears dispensable.

KW - Lysophosphatidylinositol (LPI)

KW - Glucagon-like peptide-1 (GLP-1)

KW - GPR119

KW - GPR55

KW - L-cells

KW - Mixed colonic preparation

U2 - 10.1016/j.bbalip.2018.06.007

DO - 10.1016/j.bbalip.2018.06.007

M3 - Journal article

C2 - 29883799

VL - 1863

SP - 1132

EP - 1141

JO - B B A - Molecular and Cell Biology of Lipids

JF - B B A - Molecular and Cell Biology of Lipids

SN - 1388-1981

IS - 9

ER -

ID: 212954733