Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains. / Cramer, Alyssa A.W.; Prasad, Vikram; Eftestøl, Einar; Song, Taejeong; Hansson, Kenth Arne; Dugdale, Hannah F.; Sadayappan, Sakthivel; Ochala, Julien; Gundersen, Kristian; Millay, Douglas P.

In: Nature Communications, Vol. 11, No. 1, 6287, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cramer, AAW, Prasad, V, Eftestøl, E, Song, T, Hansson, KA, Dugdale, HF, Sadayappan, S, Ochala, J, Gundersen, K & Millay, DP 2020, 'Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains', Nature Communications, vol. 11, no. 1, 6287. https://doi.org/10.1038/s41467-020-20058-7

APA

Cramer, A. A. W., Prasad, V., Eftestøl, E., Song, T., Hansson, K. A., Dugdale, H. F., Sadayappan, S., Ochala, J., Gundersen, K., & Millay, D. P. (2020). Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains. Nature Communications, 11(1), [6287]. https://doi.org/10.1038/s41467-020-20058-7

Vancouver

Cramer AAW, Prasad V, Eftestøl E, Song T, Hansson KA, Dugdale HF et al. Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains. Nature Communications. 2020;11(1). 6287. https://doi.org/10.1038/s41467-020-20058-7

Author

Cramer, Alyssa A.W. ; Prasad, Vikram ; Eftestøl, Einar ; Song, Taejeong ; Hansson, Kenth Arne ; Dugdale, Hannah F. ; Sadayappan, Sakthivel ; Ochala, Julien ; Gundersen, Kristian ; Millay, Douglas P. / Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains. In: Nature Communications. 2020 ; Vol. 11, No. 1.

Bibtex

@article{b06619b15d3f46ca9bbf7f91a113f005,
title = "Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains",
abstract = "Mammalian cells exhibit remarkable diversity in cell size, but the factors that regulate establishment and maintenance of these sizes remain poorly understood. This is especially true for skeletal muscle, comprised of syncytial myofibers that each accrue hundreds of nuclei during development. Here, we directly explore the assumed causal relationship between multinucleation and establishment of normal size through titration of myonuclear numbers during mouse neonatal development. Three independent mouse models, where myonuclear numbers were reduced by 75, 55, or 25%, led to the discovery that myonuclei possess a reserve capacity to support larger functional cytoplasmic volumes in developing myofibers. Surprisingly, the results revealed an inverse relationship between nuclei numbers and reserve capacity. We propose that as myonuclear numbers increase, the range of transcriptional return on a per nuclear basis in myofibers diminishes, which accounts for both the absolute reliance developing myofibers have on nuclear accrual to establish size, and the limits of adaptability in adult skeletal muscle.",
author = "Cramer, {Alyssa A.W.} and Vikram Prasad and Einar Eftest{\o}l and Taejeong Song and Hansson, {Kenth Arne} and Dugdale, {Hannah F.} and Sakthivel Sadayappan and Julien Ochala and Kristian Gundersen and Millay, {Douglas P.}",
year = "2020",
doi = "10.1038/s41467-020-20058-7",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Nuclear numbers in syncytial muscle fibers promote size but limit the development of larger myonuclear domains

AU - Cramer, Alyssa A.W.

AU - Prasad, Vikram

AU - Eftestøl, Einar

AU - Song, Taejeong

AU - Hansson, Kenth Arne

AU - Dugdale, Hannah F.

AU - Sadayappan, Sakthivel

AU - Ochala, Julien

AU - Gundersen, Kristian

AU - Millay, Douglas P.

PY - 2020

Y1 - 2020

N2 - Mammalian cells exhibit remarkable diversity in cell size, but the factors that regulate establishment and maintenance of these sizes remain poorly understood. This is especially true for skeletal muscle, comprised of syncytial myofibers that each accrue hundreds of nuclei during development. Here, we directly explore the assumed causal relationship between multinucleation and establishment of normal size through titration of myonuclear numbers during mouse neonatal development. Three independent mouse models, where myonuclear numbers were reduced by 75, 55, or 25%, led to the discovery that myonuclei possess a reserve capacity to support larger functional cytoplasmic volumes in developing myofibers. Surprisingly, the results revealed an inverse relationship between nuclei numbers and reserve capacity. We propose that as myonuclear numbers increase, the range of transcriptional return on a per nuclear basis in myofibers diminishes, which accounts for both the absolute reliance developing myofibers have on nuclear accrual to establish size, and the limits of adaptability in adult skeletal muscle.

AB - Mammalian cells exhibit remarkable diversity in cell size, but the factors that regulate establishment and maintenance of these sizes remain poorly understood. This is especially true for skeletal muscle, comprised of syncytial myofibers that each accrue hundreds of nuclei during development. Here, we directly explore the assumed causal relationship between multinucleation and establishment of normal size through titration of myonuclear numbers during mouse neonatal development. Three independent mouse models, where myonuclear numbers were reduced by 75, 55, or 25%, led to the discovery that myonuclei possess a reserve capacity to support larger functional cytoplasmic volumes in developing myofibers. Surprisingly, the results revealed an inverse relationship between nuclei numbers and reserve capacity. We propose that as myonuclear numbers increase, the range of transcriptional return on a per nuclear basis in myofibers diminishes, which accounts for both the absolute reliance developing myofibers have on nuclear accrual to establish size, and the limits of adaptability in adult skeletal muscle.

UR - http://www.scopus.com/inward/record.url?scp=85097259137&partnerID=8YFLogxK

U2 - 10.1038/s41467-020-20058-7

DO - 10.1038/s41467-020-20058-7

M3 - Journal article

C2 - 33293533

AN - SCOPUS:85097259137

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 6287

ER -

ID: 254993625