Neural regulation of glucagon-like peptide-1 secretion in pigs
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Neural regulation of glucagon-like peptide-1 secretion in pigs. / Hansen, Lene; Lampert, Sarah; Mineo, Hitoshi; Holst, Jens Juul.
In: American Journal of Physiology: Endocrinology and Metabolism, Vol. 287, No. 5, 11.2004, p. E939-47.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Neural regulation of glucagon-like peptide-1 secretion in pigs
AU - Hansen, Lene
AU - Lampert, Sarah
AU - Mineo, Hitoshi
AU - Holst, Jens Juul
PY - 2004/11
Y1 - 2004/11
N2 - Glucagon-like peptide (GLP)-1 is secreted rapidly from the intestine postprandially. We therefore investigated its possible neural regulation. With the use of isolated perfused porcine ileum, GLP-1 secretion was measured in response to electrical stimulation of the mixed, perivascular nerve supply and infusions of neuroactive agents alone and in combination with different blocking agents. Electrical nerve stimulation inhibited GLP-1 secretion, an effect abolished by phentolamine. Norepinephrine inhibited secretion, and phentolamine abolished this effect. GLP-1 secretion was stimulated by isoproterenol (abolished by propranolol). Acetylcholine stimulated GLP-1 secretion, and atropine blocked this effect. Dimethylphenylpiperazine stimulated GLP-1 secretion. In chloralose-anesthetized pigs, however, electrical stimulation of the vagal trunks at the level of the diaphragm had no effect on GLP-1 or GLP-2 and weak effects on glucose-dependent insulinotropic peptide and somatostatin secretion, although this elicited a marked atropine-resistant release of the neuropeptide vasoactive intestinal polypeptide to the portal circulation. Thus GLP-1 secretion is inhibited by the sympathetic nerves to the gut and may be stimulated by intrinsic cholinergic nerves, whereas the extrinsic vagal supply has no effect.
AB - Glucagon-like peptide (GLP)-1 is secreted rapidly from the intestine postprandially. We therefore investigated its possible neural regulation. With the use of isolated perfused porcine ileum, GLP-1 secretion was measured in response to electrical stimulation of the mixed, perivascular nerve supply and infusions of neuroactive agents alone and in combination with different blocking agents. Electrical nerve stimulation inhibited GLP-1 secretion, an effect abolished by phentolamine. Norepinephrine inhibited secretion, and phentolamine abolished this effect. GLP-1 secretion was stimulated by isoproterenol (abolished by propranolol). Acetylcholine stimulated GLP-1 secretion, and atropine blocked this effect. Dimethylphenylpiperazine stimulated GLP-1 secretion. In chloralose-anesthetized pigs, however, electrical stimulation of the vagal trunks at the level of the diaphragm had no effect on GLP-1 or GLP-2 and weak effects on glucose-dependent insulinotropic peptide and somatostatin secretion, although this elicited a marked atropine-resistant release of the neuropeptide vasoactive intestinal polypeptide to the portal circulation. Thus GLP-1 secretion is inhibited by the sympathetic nerves to the gut and may be stimulated by intrinsic cholinergic nerves, whereas the extrinsic vagal supply has no effect.
KW - Acetylcholine
KW - Adrenergic Agents
KW - Analysis of Variance
KW - Animals
KW - Atropine
KW - Electric Stimulation
KW - Enteric Nervous System
KW - Gastrointestinal Hormones
KW - Glucagon
KW - Glucagon-Like Peptide 1
KW - Ileum
KW - Isoproterenol
KW - Neurotransmitter Agents
KW - Norepinephrine
KW - Organ Culture Techniques
KW - Peptide Fragments
KW - Phentolamine
KW - Protein Precursors
KW - Splanchnic Nerves
KW - Swine
KW - Vagus Nerve
U2 - 10.1152/ajpendo.00197.2004
DO - 10.1152/ajpendo.00197.2004
M3 - Journal article
C2 - 15475512
VL - 287
SP - E939-47
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 5
ER -
ID: 132054137