Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses?

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses? / Stavnsbjerg, Camilla; Jørgensen, Jennifer Solgaard; Engel, Trine Bjørnbo; Brus, Anja; Ringgaard, Lars; Hansen, Anders Elias; Kjaer, Andreas; Andresen, Thomas Lars.

In: Journal of Immunological Methods, Vol. 500, 113177, 2022, p. 1-4.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Stavnsbjerg, C, Jørgensen, JS, Engel, TB, Brus, A, Ringgaard, L, Hansen, AE, Kjaer, A & Andresen, TL 2022, 'Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses?', Journal of Immunological Methods, vol. 500, 113177, pp. 1-4. https://doi.org/10.1016/j.jim.2021.113177

APA

Stavnsbjerg, C., Jørgensen, J. S., Engel, T. B., Brus, A., Ringgaard, L., Hansen, A. E., Kjaer, A., & Andresen, T. L. (2022). Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses? Journal of Immunological Methods, 500, 1-4. [113177]. https://doi.org/10.1016/j.jim.2021.113177

Vancouver

Stavnsbjerg C, Jørgensen JS, Engel TB, Brus A, Ringgaard L, Hansen AE et al. Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses? Journal of Immunological Methods. 2022;500:1-4. 113177. https://doi.org/10.1016/j.jim.2021.113177

Author

Stavnsbjerg, Camilla ; Jørgensen, Jennifer Solgaard ; Engel, Trine Bjørnbo ; Brus, Anja ; Ringgaard, Lars ; Hansen, Anders Elias ; Kjaer, Andreas ; Andresen, Thomas Lars. / Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses?. In: Journal of Immunological Methods. 2022 ; Vol. 500. pp. 1-4.

Bibtex

@article{43237634c6c447e79ad05c8bcc6ea7bc,
title = "Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses?",
abstract = "Quantification of cytokines in cancerous tissue is important for understanding basic tumor biology and for deciphering anti-cancer mechanisms in drug development. Cytokine measurements on protein-level are often done by immunoassays such as enzyme-linked immunosorbent assay (ELISAs) and multiplex assays. However, immunoassays are prone to interference due to the presence of perturbing factors. The sum of these factors is known as the matrix effect, which results in a deviation of the measured cytokine concentration from the actual concentration. In this study, we demonstrated that matrix effects are present in tumor lysates from 11 different syngeneic murine tumors and that it can greatly affect cytokine measurements in ELISAs and multiplex assays. Dilution of tumor lysates and careful selection of lysis buffer components may decrease matrix effects. However, matrix effects are still present, and care should be taken when analyzing cytokine measurements of tumor lysates.",
author = "Camilla Stavnsbjerg and J{\o}rgensen, {Jennifer Solgaard} and Engel, {Trine Bj{\o}rnbo} and Anja Brus and Lars Ringgaard and Hansen, {Anders Elias} and Andreas Kjaer and Andresen, {Thomas Lars}",
year = "2022",
doi = "10.1016/j.jim.2021.113177",
language = "English",
volume = "500",
pages = "1--4",
journal = "Journal of Immunological Methods",
issn = "0022-1759",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Matrix effect in tumor lysates – Does it affect your cytokine ELISA and multiplex analyses?

AU - Stavnsbjerg, Camilla

AU - Jørgensen, Jennifer Solgaard

AU - Engel, Trine Bjørnbo

AU - Brus, Anja

AU - Ringgaard, Lars

AU - Hansen, Anders Elias

AU - Kjaer, Andreas

AU - Andresen, Thomas Lars

PY - 2022

Y1 - 2022

N2 - Quantification of cytokines in cancerous tissue is important for understanding basic tumor biology and for deciphering anti-cancer mechanisms in drug development. Cytokine measurements on protein-level are often done by immunoassays such as enzyme-linked immunosorbent assay (ELISAs) and multiplex assays. However, immunoassays are prone to interference due to the presence of perturbing factors. The sum of these factors is known as the matrix effect, which results in a deviation of the measured cytokine concentration from the actual concentration. In this study, we demonstrated that matrix effects are present in tumor lysates from 11 different syngeneic murine tumors and that it can greatly affect cytokine measurements in ELISAs and multiplex assays. Dilution of tumor lysates and careful selection of lysis buffer components may decrease matrix effects. However, matrix effects are still present, and care should be taken when analyzing cytokine measurements of tumor lysates.

AB - Quantification of cytokines in cancerous tissue is important for understanding basic tumor biology and for deciphering anti-cancer mechanisms in drug development. Cytokine measurements on protein-level are often done by immunoassays such as enzyme-linked immunosorbent assay (ELISAs) and multiplex assays. However, immunoassays are prone to interference due to the presence of perturbing factors. The sum of these factors is known as the matrix effect, which results in a deviation of the measured cytokine concentration from the actual concentration. In this study, we demonstrated that matrix effects are present in tumor lysates from 11 different syngeneic murine tumors and that it can greatly affect cytokine measurements in ELISAs and multiplex assays. Dilution of tumor lysates and careful selection of lysis buffer components may decrease matrix effects. However, matrix effects are still present, and care should be taken when analyzing cytokine measurements of tumor lysates.

U2 - 10.1016/j.jim.2021.113177

DO - 10.1016/j.jim.2021.113177

M3 - Journal article

C2 - 34756881

VL - 500

SP - 1

EP - 4

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

SN - 0022-1759

M1 - 113177

ER -

ID: 283130190