Isolation, characterization, and enzymatic hydrolysis of acetyl-galactoglucomannan from spruce (Picea abies)
Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review
Water-soluble hemicelluloses were extracted from spruce chips by heat-fractionation using microwave treatment. A screening of conditions (pH, temperature and residence time) was performed for the extraction of O-acetyl-galactoglucomannan (AcGGM). The yield and the average molecular weight of the extracted mannan were analysed using HPLC, size-exclusion chromatography (SEC) and mass-spectrometry. The pH during heat fractionation influenced the yield and the structure of AcGGM. The highest yield (78%) of AcGGM (average molecular weight 3800) was achieved with heat-fractionation in water at 190° C for 5 minutes. With 0.025% NaOH, an average molecular weight of 9500 was obtained at a yield of 30%. AcGGM molecular weight standard molecules were prepared using SEC. The structure of the AcGGM was determined using 1H-NMR. The enzymatic hydrolysis of AcGGM was studied using β-mannanase and α-galactosidase.
Original language | English |
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Title of host publication | Henicelluloses : Science and Technology |
Editors | Paul Gatenholm, Maija Tenkanen |
Number of pages | 13 |
Publication date | 1 Dec 2004 |
Pages | 66-78 |
ISBN (Print) | 9780841238428 |
Publication status | Published - 1 Dec 2004 |
Series | A C S Symposium Series |
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Volume | 864 |
ISSN | 0097-6156 |
ID: 240161930