Increased cellular proliferation in rat skeletal muscle and tendon in response to exercise: use of FLT and PET/CT
Research output: Contribution to journal › Journal article › Research › peer-review
PURPOSE: The purpose of this study is to investigate exercise-induced cellular proliferation in rat skeletal muscle/tendon with the use of 3'-[F-18]fluoro-3'deoxythymidine (FLT) and to quantitatively study concomitant changes in the proliferation-associated factor, Ki67.
PROCEDURES: Wistar rats (n = 13) performed 3 days of treadmill running. Cellular proliferation was investigated 3 days before and 48 h after the running exercise with the use of FLT and positron emission tomography/computed tomography (PET/CT). Results were compared to a sedentary control group (n = 10). Image-derived standardized uptake values were calculated for Achilles tendons and calf muscles and compared to gene expression and immunohistochemical evaluations of Ki67.
RESULTS: Treadmill running induced increased uptake of FLT uptake in calf muscles (30%; p < 0.001) and in Achilles tendon (21%, p < 0.001). The image-derived results were supported by a correlation in calf muscle to Ki67 (protein and mRNA level), while this coherence was not found in tendon.
CONCLUSION: FLT-PET seems to be a promising tool for imaging of exercise-induced cellular proliferation in musculo-tendinous tissue.
Original language | English |
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Journal | Molecular Imaging and Biology |
Volume | 12 |
Issue number | 6 |
Pages (from-to) | 626-634 |
Number of pages | 9 |
ISSN | 1536-1632 |
DOIs | |
Publication status | Published - 2010 |
- Animals, Cell Proliferation, Dideoxynucleosides, Exercise Test, Hyperplasia, Ki-67 Antigen, Male, Muscle, Skeletal, Physical Conditioning, Animal, Positron-Emission Tomography, Rats, Rats, Wistar, Running, Tendons, Tomography, Emission-Computed, Up-Regulation
Research areas
ID: 34113802