Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons

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Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons. / Tal, D M; Yanuck, M D; Van Hall, Gerrit; Karlish, S J.

In: BBA General Subjects, Vol. 985, No. 1, 1989, p. 55-9.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Tal, DM, Yanuck, MD, Van Hall, G & Karlish, SJ 1989, 'Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons', BBA General Subjects, vol. 985, no. 1, pp. 55-9.

APA

Tal, D. M., Yanuck, M. D., Van Hall, G., & Karlish, S. J. (1989). Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons. BBA General Subjects, 985(1), 55-9.

Vancouver

Tal DM, Yanuck MD, Van Hall G, Karlish SJ. Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons. BBA General Subjects. 1989;985(1):55-9.

Author

Tal, D M ; Yanuck, M D ; Van Hall, Gerrit ; Karlish, S J. / Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons. In: BBA General Subjects. 1989 ; Vol. 985, No. 1. pp. 55-9.

Bibtex

@article{f6e755504f7411de87b8000ea68e967b,
title = "Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons",
abstract = "A preparative purification of endogenous inhibitors of the Na+/K+-ATPase has been carried out from bovine blood. Dried plasma was deproteinized, hexane-extracted and desalted, followed by further purification through a series of reverse-phase HPLC fractionations. Fractions active in inhibiting Na+/K+-ATPase activity and displacing ouabain were collected and purified further. By comparison with ouabain, the final extract was found to have a steeper concentration-effect curve in the inhibition of Na+/K+-ATPase. In displacement of [3H]ouabain, the extract had again a steeper concentration-effect curve than does ouabain, and in addition it enhanced ouabain binding at high dilutions. These properties are indicative of nonspecific interactions with the Na+/K+-ATPase. The active fraction was identified by TLC, HPLC, NMR, GLC and GC-MS, to be a mixture of three unesterified fatty acids, mainly oleic acid (72% of the total) and three saturated hydrocarbons. The assignment of structures was corroborated by comparison with authentic samples.",
author = "Tal, {D M} and Yanuck, {M D} and {Van Hall}, Gerrit and Karlish, {S J}",
note = "Keywords: Animals; Cattle; Chromatography, Gas; Chromatography, High Pressure Liquid; Enzyme Inhibitors; Fatty Acids; Gas Chromatography-Mass Spectrometry; Hydrocarbons; Kidney Medulla; Ouabain; Sodium-Potassium-Exchanging ATPase; Swine",
year = "1989",
language = "English",
volume = "985",
pages = "55--9",
journal = "B B A - General Subjects",
issn = "0304-4165",
publisher = "Elsevier",
number = "1",

}

RIS

TY - JOUR

T1 - Identification of Na+/K+-ATPase inhibitors in bovine plasma as fatty acids and hydrocarbons

AU - Tal, D M

AU - Yanuck, M D

AU - Van Hall, Gerrit

AU - Karlish, S J

N1 - Keywords: Animals; Cattle; Chromatography, Gas; Chromatography, High Pressure Liquid; Enzyme Inhibitors; Fatty Acids; Gas Chromatography-Mass Spectrometry; Hydrocarbons; Kidney Medulla; Ouabain; Sodium-Potassium-Exchanging ATPase; Swine

PY - 1989

Y1 - 1989

N2 - A preparative purification of endogenous inhibitors of the Na+/K+-ATPase has been carried out from bovine blood. Dried plasma was deproteinized, hexane-extracted and desalted, followed by further purification through a series of reverse-phase HPLC fractionations. Fractions active in inhibiting Na+/K+-ATPase activity and displacing ouabain were collected and purified further. By comparison with ouabain, the final extract was found to have a steeper concentration-effect curve in the inhibition of Na+/K+-ATPase. In displacement of [3H]ouabain, the extract had again a steeper concentration-effect curve than does ouabain, and in addition it enhanced ouabain binding at high dilutions. These properties are indicative of nonspecific interactions with the Na+/K+-ATPase. The active fraction was identified by TLC, HPLC, NMR, GLC and GC-MS, to be a mixture of three unesterified fatty acids, mainly oleic acid (72% of the total) and three saturated hydrocarbons. The assignment of structures was corroborated by comparison with authentic samples.

AB - A preparative purification of endogenous inhibitors of the Na+/K+-ATPase has been carried out from bovine blood. Dried plasma was deproteinized, hexane-extracted and desalted, followed by further purification through a series of reverse-phase HPLC fractionations. Fractions active in inhibiting Na+/K+-ATPase activity and displacing ouabain were collected and purified further. By comparison with ouabain, the final extract was found to have a steeper concentration-effect curve in the inhibition of Na+/K+-ATPase. In displacement of [3H]ouabain, the extract had again a steeper concentration-effect curve than does ouabain, and in addition it enhanced ouabain binding at high dilutions. These properties are indicative of nonspecific interactions with the Na+/K+-ATPase. The active fraction was identified by TLC, HPLC, NMR, GLC and GC-MS, to be a mixture of three unesterified fatty acids, mainly oleic acid (72% of the total) and three saturated hydrocarbons. The assignment of structures was corroborated by comparison with authentic samples.

M3 - Journal article

C2 - 2551389

VL - 985

SP - 55

EP - 59

JO - B B A - General Subjects

JF - B B A - General Subjects

SN - 0304-4165

IS - 1

ER -

ID: 12485088