High-yield overproduction and purification of human aquaporins from Pichia pastoris

Research output: Contribution to journal › Journal article › Research › peer-review

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High-yield overproduction and purification of human aquaporins from Pichia pastoris. / Al-Jubair, Tamim; Steffen, Jonas Hyld; Missel, Julie Winkel; Kitchen, Philip; Salman, Mootaz M.; Bill, Roslyn M.; Gourdon, Pontus; Törnroth-Horsefield, Susanna.

In: STAR Protocols, Vol. 3, No. 2, 101298, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Al-Jubair, T, Steffen, JH, Missel, JW, Kitchen, P, Salman, MM, Bill, RM, Gourdon, P & Törnroth-Horsefield, S 2022, 'High-yield overproduction and purification of human aquaporins from Pichia pastoris', STAR Protocols, vol. 3, no. 2, 101298. https://doi.org/10.1016/j.xpro.2022.101298

APA

Al-Jubair, T., Steffen, J. H., Missel, J. W., Kitchen, P., Salman, M. M., Bill, R. M., Gourdon, P., & Törnroth-Horsefield, S. (2022). High-yield overproduction and purification of human aquaporins from Pichia pastoris. STAR Protocols, 3(2), [101298]. https://doi.org/10.1016/j.xpro.2022.101298

Vancouver

Al-Jubair T, Steffen JH, Missel JW, Kitchen P, Salman MM, Bill RM et al. High-yield overproduction and purification of human aquaporins from Pichia pastoris. STAR Protocols. 2022;3(2). 101298. https://doi.org/10.1016/j.xpro.2022.101298

Author

Al-Jubair, Tamim ; Steffen, Jonas Hyld ; Missel, Julie Winkel ; Kitchen, Philip ; Salman, Mootaz M. ; Bill, Roslyn M. ; Gourdon, Pontus ; Törnroth-Horsefield, Susanna. / High-yield overproduction and purification of human aquaporins from Pichia pastoris. In: STAR Protocols. 2022 ; Vol. 3, No. 2.

Bibtex

@article{1e4846fe19de4cefa83c43aaa92eb40b,
title = "High-yield overproduction and purification of human aquaporins from Pichia pastoris",
abstract = "Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia pastoris and subsequent AQP purification. The protocol typically yields 1–5 mg AQP per g of yeast cell at >95% purity and is compatible with any membrane protein cloned into Pichia pastoris, although expression levels may vary. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020) and Frick et al. (2014).",
keywords = "Cell Membrane, Molecular Biology, Protein Biochemistry, Protein expression and purification",
author = "Tamim Al-Jubair and Steffen, {Jonas Hyld} and Missel, {Julie Winkel} and Philip Kitchen and Salman, {Mootaz M.} and Bill, {Roslyn M.} and Pontus Gourdon and Susanna T{\"o}rnroth-Horsefield",
note = "Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
doi = "10.1016/j.xpro.2022.101298",
language = "English",
volume = "3",
journal = "STAR Protocols",
issn = "2666-1667",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - High-yield overproduction and purification of human aquaporins from Pichia pastoris

AU - Al-Jubair, Tamim

AU - Steffen, Jonas Hyld

AU - Missel, Julie Winkel

AU - Kitchen, Philip

AU - Salman, Mootaz M.

AU - Bill, Roslyn M.

AU - Gourdon, Pontus

AU - Törnroth-Horsefield, Susanna

N1 - Publisher Copyright: © 2022 The Authors

PY - 2022

Y1 - 2022

N2 - Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia pastoris and subsequent AQP purification. The protocol typically yields 1–5 mg AQP per g of yeast cell at >95% purity and is compatible with any membrane protein cloned into Pichia pastoris, although expression levels may vary. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020) and Frick et al. (2014).

AB - Aquaporins (AQPs) are membrane-bound water channels that play crucial roles in maintaining the water homeostasis of the human body. Here, we present a protocol for high-yield recombinant expression of human AQPs in the methylotropic yeast Pichia pastoris and subsequent AQP purification. The protocol typically yields 1–5 mg AQP per g of yeast cell at >95% purity and is compatible with any membrane protein cloned into Pichia pastoris, although expression levels may vary. For complete details on the use and execution of this protocol, please refer to Kitchen et al. (2020) and Frick et al. (2014).

KW - Cell Membrane

KW - Molecular Biology

KW - Protein Biochemistry

KW - Protein expression and purification

UR - http://www.scopus.com/inward/record.url?scp=85127974289&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2022.101298

DO - 10.1016/j.xpro.2022.101298

M3 - Journal article

C2 - 35463467

AN - SCOPUS:85127974289

VL - 3

JO - STAR Protocols

JF - STAR Protocols

SN - 2666-1667

IS - 2

M1 - 101298

ER -

ID: 311126156