Expression Profile of the GLP-1 Receptor in the Gastrointestinal Tract and Pancreas in Adult Female Mice

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Expression Profile of the GLP-1 Receptor in the Gastrointestinal Tract and Pancreas in Adult Female Mice. / Grunddal, Kaare V.; Jensen, Elisa P.; Ørskov, Cathrine; Andersen, Daniel B.; Windeløv, Johanne A.; Poulsen, Steen Seier; Rosenkilde, Mette M; Knudsen, Lotte Bjerre; Pyke, Charles; Holst, Jens J.

In: Endocrinology, Vol. 163, No. 1, 2022.

Research output: Contribution to journalJournal articlepeer-review

Harvard

Grunddal, KV, Jensen, EP, Ørskov, C, Andersen, DB, Windeløv, JA, Poulsen, SS, Rosenkilde, MM, Knudsen, LB, Pyke, C & Holst, JJ 2022, 'Expression Profile of the GLP-1 Receptor in the Gastrointestinal Tract and Pancreas in Adult Female Mice', Endocrinology, vol. 163, no. 1. https://doi.org/10.1210/endocr/bqab216

APA

Grunddal, K. V., Jensen, E. P., Ørskov, C., Andersen, D. B., Windeløv, J. A., Poulsen, S. S., Rosenkilde, M. M., Knudsen, L. B., Pyke, C., & Holst, J. J. (2022). Expression Profile of the GLP-1 Receptor in the Gastrointestinal Tract and Pancreas in Adult Female Mice. Endocrinology, 163(1). https://doi.org/10.1210/endocr/bqab216

Vancouver

Grunddal KV, Jensen EP, Ørskov C, Andersen DB, Windeløv JA, Poulsen SS et al. Expression Profile of the GLP-1 Receptor in the Gastrointestinal Tract and Pancreas in Adult Female Mice. Endocrinology. 2022;163(1). https://doi.org/10.1210/endocr/bqab216

Author

Grunddal, Kaare V. ; Jensen, Elisa P. ; Ørskov, Cathrine ; Andersen, Daniel B. ; Windeløv, Johanne A. ; Poulsen, Steen Seier ; Rosenkilde, Mette M ; Knudsen, Lotte Bjerre ; Pyke, Charles ; Holst, Jens J. / Expression Profile of the GLP-1 Receptor in the Gastrointestinal Tract and Pancreas in Adult Female Mice. In: Endocrinology. 2022 ; Vol. 163, No. 1.

Bibtex

@article{20325c85d87f46d7b274c5add48b9f1c,
title = "Expression Profile of the GLP-1 Receptor in the Gastrointestinal Tract and Pancreas in Adult Female Mice",
abstract = "Therapies based on glucagon-like peptide-1 receptor (GLP-1R) agonism are highly effective in treating type 2 diabetes and obesity, but the localization of GLP-1Rs mediating the antidiabetic and other possible actions of GLP-1 is still debated. The purpose with this study was to identify sites of GLP-1R mRNA and protein expression in the mouse gastrointestinal system by means of GLP-1R antibody immunohistochemistry, Glp1r mRNA fluorescence in situ hybridization, and 125I-exendin (9-39) autoradiography. As expected, GLP-1R staining was observed in almost all β-cells in the pancreatic islets, but more rarely in α- and δ-cells. In the stomach, GLP-1R staining was found exclusively in the gastric corpus mucous neck cells, known to protect the stomach mucosa. The Brunner glands were strongly stained for GLP-1R, and pretreatment with GLP-1 agonist exendin-4 caused internalization of the receptor and mucin secretion, while pretreatment with phosphate-buffered saline or antagonist exendin (9-39) did not. In the intestinal mucosa, GLP-1R staining was observed in intraepithelial lymphocytes, lamina propria lymphocytes, and enteroendocrine cells containing secretin, peptide YY, and somatostatin, but not cholecystokinin. GLP-1R staining was seen in nerve fibers within the choline acetyl transferase- and nitric oxide-positive myenteric plexuses from the gastric corpus to the distal large intestine being strongest in the mid- and hindgut area. Finally, intraperitoneal administration of radiolabeled exendin (9-39) strongly labeled myenteric fibers. In conclusion, this study expands our knowledge of GLP-1R localization and suggests that GLP-1 may serve an important role in modulating gastrointestinal health and mucosal protection.",
author = "Grunddal, {Kaare V.} and Jensen, {Elisa P.} and Cathrine {\O}rskov and Andersen, {Daniel B.} and Windel{\o}v, {Johanne A.} and Poulsen, {Steen Seier} and Rosenkilde, {Mette M} and Knudsen, {Lotte Bjerre} and Charles Pyke and Holst, {Jens J.}",
note = "{\textcopyright} The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society.",
year = "2022",
doi = "10.1210/endocr/bqab216",
language = "English",
volume = "163",
journal = "Journal of Clinical Endocrinology and Metabolism",
issn = "0013-7227",
publisher = "Oxford University Press",
number = "1",

}

RIS

TY - JOUR

T1 - Expression Profile of the GLP-1 Receptor in the Gastrointestinal Tract and Pancreas in Adult Female Mice

AU - Grunddal, Kaare V.

AU - Jensen, Elisa P.

AU - Ørskov, Cathrine

AU - Andersen, Daniel B.

AU - Windeløv, Johanne A.

AU - Poulsen, Steen Seier

AU - Rosenkilde, Mette M

AU - Knudsen, Lotte Bjerre

AU - Pyke, Charles

AU - Holst, Jens J.

N1 - © The Author(s) 2021. Published by Oxford University Press on behalf of the Endocrine Society.

PY - 2022

Y1 - 2022

N2 - Therapies based on glucagon-like peptide-1 receptor (GLP-1R) agonism are highly effective in treating type 2 diabetes and obesity, but the localization of GLP-1Rs mediating the antidiabetic and other possible actions of GLP-1 is still debated. The purpose with this study was to identify sites of GLP-1R mRNA and protein expression in the mouse gastrointestinal system by means of GLP-1R antibody immunohistochemistry, Glp1r mRNA fluorescence in situ hybridization, and 125I-exendin (9-39) autoradiography. As expected, GLP-1R staining was observed in almost all β-cells in the pancreatic islets, but more rarely in α- and δ-cells. In the stomach, GLP-1R staining was found exclusively in the gastric corpus mucous neck cells, known to protect the stomach mucosa. The Brunner glands were strongly stained for GLP-1R, and pretreatment with GLP-1 agonist exendin-4 caused internalization of the receptor and mucin secretion, while pretreatment with phosphate-buffered saline or antagonist exendin (9-39) did not. In the intestinal mucosa, GLP-1R staining was observed in intraepithelial lymphocytes, lamina propria lymphocytes, and enteroendocrine cells containing secretin, peptide YY, and somatostatin, but not cholecystokinin. GLP-1R staining was seen in nerve fibers within the choline acetyl transferase- and nitric oxide-positive myenteric plexuses from the gastric corpus to the distal large intestine being strongest in the mid- and hindgut area. Finally, intraperitoneal administration of radiolabeled exendin (9-39) strongly labeled myenteric fibers. In conclusion, this study expands our knowledge of GLP-1R localization and suggests that GLP-1 may serve an important role in modulating gastrointestinal health and mucosal protection.

AB - Therapies based on glucagon-like peptide-1 receptor (GLP-1R) agonism are highly effective in treating type 2 diabetes and obesity, but the localization of GLP-1Rs mediating the antidiabetic and other possible actions of GLP-1 is still debated. The purpose with this study was to identify sites of GLP-1R mRNA and protein expression in the mouse gastrointestinal system by means of GLP-1R antibody immunohistochemistry, Glp1r mRNA fluorescence in situ hybridization, and 125I-exendin (9-39) autoradiography. As expected, GLP-1R staining was observed in almost all β-cells in the pancreatic islets, but more rarely in α- and δ-cells. In the stomach, GLP-1R staining was found exclusively in the gastric corpus mucous neck cells, known to protect the stomach mucosa. The Brunner glands were strongly stained for GLP-1R, and pretreatment with GLP-1 agonist exendin-4 caused internalization of the receptor and mucin secretion, while pretreatment with phosphate-buffered saline or antagonist exendin (9-39) did not. In the intestinal mucosa, GLP-1R staining was observed in intraepithelial lymphocytes, lamina propria lymphocytes, and enteroendocrine cells containing secretin, peptide YY, and somatostatin, but not cholecystokinin. GLP-1R staining was seen in nerve fibers within the choline acetyl transferase- and nitric oxide-positive myenteric plexuses from the gastric corpus to the distal large intestine being strongest in the mid- and hindgut area. Finally, intraperitoneal administration of radiolabeled exendin (9-39) strongly labeled myenteric fibers. In conclusion, this study expands our knowledge of GLP-1R localization and suggests that GLP-1 may serve an important role in modulating gastrointestinal health and mucosal protection.

U2 - 10.1210/endocr/bqab216

DO - 10.1210/endocr/bqab216

M3 - Journal article

C2 - 34662392

VL - 163

JO - Journal of Clinical Endocrinology and Metabolism

JF - Journal of Clinical Endocrinology and Metabolism

SN - 0013-7227

IS - 1

ER -

ID: 287119198