EPR spin trapping of protein radicals

Research output: Contribution to journalJournal articleResearchpeer-review

Electron paramagnetic resonance (EPR) spin trapping was originally developed to aid the detection of low-molecular-mass radicals formed in chemical systems. It has subsequently found widespread use in biology and medicine for the direct detection of radical species formed during oxidative stress and via enzymatic reactions. Over the last 15 years this technique has also found increasing use in detecting and identifying radicals formed on biological macromolecules as a result of either radical reactions or enzymatic processes. Though the EPR signals that result from the trapping of large, slowly tumbling radicals are often broad and relatively poor in distinctive features, a number of techniques have been developed that allow a wealth of information to be obtained about the nature, site, and reactions of such radicals. This article summarizes recent developments in this area and reviews selected examples of radical formation on proteins.

Original languageEnglish
JournalFree Radical Biology & Medicine
Issue number9
Pages (from-to)1072-86
Number of pages15
Publication statusPublished - 2004

    Research areas

  • Artifacts, Electron Spin Resonance Spectroscopy, Free Radicals, Oxidation-Reduction, Proteins, Spin Trapping

ID: 138273704