Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements

Research output: Contribution to journal › Journal article › peer-review

  • Lundby, Alicia
  • Hiroki Mutoh
  • Dimitar Dimitrov
  • Walther Akemann
  • Thomas Knöpfel
Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.
Original languageEnglish
JournalPLoS ONE
Volume3
Issue number6
Pages (from-to)e2514
ISSN1932-6203
DOIs
Publication statusPublished - 2008

Bibliographical note

Keywords: Animals; Fluorescence; Genetic Engineering; Ion Channel Gating; PC12 Cells; Potassium Channels; Protein Conformation; Rats

ID: 20969902