Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements
Research output: Contribution to journal › Journal article › Research › peer-review
Ci-VSP contains a voltage-sensing domain (VSD) homologous to that of voltage-gated potassium channels. Using charge displacement ('gating' current) measurements we show that voltage-sensing movements of this VSD can occur within 1 ms in mammalian membranes. Our analysis lead to development of a genetically encodable fluorescent protein voltage sensor (VSFP) in which the fast, voltage-dependent conformational changes of the Ci-VSP voltage sensor are transduced to similarly fast fluorescence read-outs.
|Publication status||Published - 2008|
Keywords: Animals; Fluorescence; Genetic Engineering; Ion Channel Gating; PC12 Cells; Potassium Channels; Protein Conformation; Rats